LOCUS       Exported               10292 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pEMB018 / LRRK2_BC_1185_2136
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6342..6359
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     6396..7753
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(6412..7753)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7845..7862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8016..8604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8096..8115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8775..9635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9398..9417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9636..9740)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9767..10222
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9854..9873)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10064..10085
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccttctcgtg catcccagag
     3481 gcaatcctca acctgcctca cctgcgtagc ctggacatga gttccaatga catccagtac
     3541 ctgcccggtc ctgcccactg gaagtcattg aatctgcgtg aactcctgtt ctcccataat
     3601 cagatttcca tcctggacct ttcagagaag gcctacctct ggtctcgcgt ggaaaagcta
     3661 catctaagtc acaacaagct gaaggaaatc cctcctgaga tcgggtgcct ggaaaacctg
     3721 acctccttgg acgtctctta taacctggaa ctgcgttcct ttcctaacga aatgggcaaa
     3781 ctctctaaga tttgggacct gcctctggac gagctgcacc taaacttcga cttcaagcac
     3841 attggctgta aagcaaagga tattattcgt ttcctgcagc agcgtcttaa gaaggccgtg
     3901 ccctacaatc gcatgaagct gatgatcgtc gggaacaccg gttctggaaa gacaaccctc
     3961 ttgcagcagc ttatgaagac taagaagtcc gacttgggga tgcagagcgc cactgtgggg
     4021 atcgacgtga aagactggcc aatccaaatc cgtgacaagc gcaagcgtga cttagtactg
     4081 aacgtgtggg actttgccgg cagggaagag ttctactcta cgcatcctca cttcatgact
     4141 cagcgtgccc tctatctggc agtttacgac ctctcaaagg gccaggctga ggtggacgcc
     4201 atgaaaccct ggctgtttaa catcaaggct cgtgcttcca gctcccctgt gatcttagtc
     4261 gggacacact tggatgtttc tgatgagaag cagcgtaagg catgtatgag taagatcacc
     4321 aaagagctgt tgaacaaacg cggctttccc gccatccgtg attaccactt cgtcaacgct
     4381 accgaggaaa gcgacgcact ggccaagtta cgtaagacca ttatcaacga aagcctgaac
     4441 ttcaaaattc gcgaccagct ggtggtcgga cagctgatcc ccgactgcta cgtggaactt
     4501 gagaagatca tcctgagcga gcgcaagaat gtgcctatcg agtttccagt gatcgatcgc
     4561 aaacgtctcc tccagctcgt gcgtgagaat cagctgcagc tcgacgagaa cgagctgcct
     4621 catgctgtgc acttccttaa cgagtcaggt gtcctcctgc acttccagga cccggctctc
     4681 cagctgagtg acctctattt cgtggagcca aagtggctct gtaaaattat ggctcaaatc
     4741 ctcactgtca aggtagaggg ctgtccaaag cacccaaagg gcatcatctc gcgtcgcgac
     4801 gtggaaaagt ttctctctaa gaagcgcaag tttcccaaga actacatgag ccagtacttt
     4861 aaactgttgg agaagttcca gatcgctctg cctatcggcg aagagtacct gttggttcct
     4921 tcctccctgt ccgatcaccg ccccgtgatt gagctgcctc actgcgagaa cagcgagatt
     4981 atcattcgcc tatacgagat gccgtacttt ccgatgggct tctggtcaag gctcatcaat
     5041 cgtctgcttg aaatctcccc atatatgctg agcggtcgtg aacgcgctct gaggccaaac
     5101 cggatgtact ggcgccaggg gatttacttg aactggtccc ctgaggctta ttgtctggtg
     5161 ggctcggaag tcctggataa ccaccccgaa tcattcctga agatcaccgt tccttcctgc
     5221 cgtaagggtt gcattctatt gggacaggtg gtggatcata tcgattccct gatggaggag
     5281 tggttccctg gcctgttgga gatcgacatc tgtggcgagg gtgagaccct gctgaagaag
     5341 tgggcactct acagtttcaa tgacggcgaa gaacaccaga agattctgct cgacgacctc
     5401 atgaagaagg ccgaggaggg cgacctgctc gtgaaccccg accagcctag gctgaccatc
     5461 cccatctccc agattgcacc cgaccttatc ctcgctgacc tgcctcgcaa catcatgctg
     5521 aacaatgacg agctggagtt tgaacaagca cccgagttcc tgctaggcga cggctctttc
     5581 ggttcagttt accgtgctgc ttacgagggt gaagaggttg ccgtgaagat ttttaacaag
     5641 cacacttcgc tccgtctgct gcgtcaggag cttgtggttc tctgccacct gcaccaccct
     5701 tccctgatct ccctgctggc cgccggcatt aggcctcgca tgctagtgat ggaactggcc
     5761 agtaagggtt cattggaccg cctgctgcag caggacaagg ctagcctgac ccgtacgctc
     5821 cagcaccgta tcgccctgca tgtggccgac ggactgcgct acttgcacag tgccatgatc
     5881 atttatcgcg atctgaagcc tcataatgtt cttctgttca cgctctaccc aaacgcagct
     5941 atcattgcca aaattgccga ttacggcata gcccagtact gctgtcgtat gggtatcaag
     6001 actagcgagg gtacacctgg gttccgcgct cccgaagtcg cacgcgggaa cgtgatctac
     6061 aaccagcagg ccgacgtgta ctcgtttggc ctgctcctgt atgacattct cactaccgga
     6121 ggccggattg tggagggcct gaaatttccc aacgagttcg acgagctgga gatccagggc
     6181 aagctgcctg accccgtgaa ggagtacggt tgtgcaccct ggcctatggt ggagaagctc
     6241 attaagcagt gtctgaagga gaaccctcag gaacgcccca caagcgccca ggtgttcgac
     6301 attctaaact ctgcagagta aagcttgcgg ccgcactcga gcaccaccac caccaccact
     6361 aacctaggta gctgagcgca tgcaagctga tccgggttat tagtacattt attaagcgct
     6421 agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa
     6481 tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata
     6541 tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa
     6601 caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac
     6661 aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt
     6721 ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact
     6781 gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac
     6841 atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga
     6901 agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa
     6961 cacgtccgcg atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt
     7021 ttgggcgggt ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga
     7081 tggtgcaggc ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc
     7141 tgatgataaa tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg
     7201 aggtggtggc ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt
     7261 cggcacctca actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg
     7321 agtgcgattt ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc
     7381 agcgggttga ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg
     7441 tggtggtggt ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc
     7501 cggtataatt tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc
     7561 cgctggctgc acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc
     7621 aatattataa ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc
     7681 gtttaccgtg ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg
     7741 tctcaagctc ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat
     7801 tgtagtggcg agacacttcg ctgtcgtcga ggtttaaacg cttcctcgct cactgactcg
     7861 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7921 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7981 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8041 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8101 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8161 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8221 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8281 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8341 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8401 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8461 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8521 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8581 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8641 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8701 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8761 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8821 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8881 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8941 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9001 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9061 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9121 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9181 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9241 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9301 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9361 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9421 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9481 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9541 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9601 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9661 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9721 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     9781 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9841 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9901 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9961 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10021 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10081 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10141 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10201 aaaatattaa cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg
    10261 cgatcggtgc gggcctcttc gctattacgc ca
//
