LOCUS       Exported                 838 bp ds-DNA     linear   SYN 28-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    LRRK2_1867_2136
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 838)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..120
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             124..777
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
ORIGIN
        1 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccacg agaaccttta tttccaagga
      121 tcaatggcac cgatcctggg gtattggaag atcaagggcc tagtgcagcc cactcgtctg
      181 ctcctggagt acctagaaga gaagtatgag gagcaccttt atgagcggga cgagggtgac
      241 aagtggcgca ataagaaatt cgagctaggc ttggaattcc ccaatctgcc ttattacatc
      301 gatggcgacg ttaagttgac tcagtccatg gccatcatcc gctacatcgc cgacaagcac
      361 aatatgctag gcgggtgccc gaaggagcga gctgagatct ctatgttgga aggggccgtg
      421 ctggatatcc gctacggcgt gagccgcatt gcatactcaa aggatttcga gaccctcaag
      481 gttgacttcc tgtcaaagct gcctgaaatg ctaaagatgt tcgaagaccg tctctgtcat
      541 aagacctacc tgaacgggga ccacgtgaca cacccggact ttatgttgta tgacgctctg
      601 gacgtcgtgc tttatatgga ccctatgtgc ctggacgctt ttcccaaact agtgtgtttc
      661 aagaaacgta tcgaagctat ccctcagatt gataagtacc tgaagtcctc aaagtacatc
      721 gcctggcccc ttcaaggttg gcaagccaca tttggcggtg gcgaccatcc tcccaagtca
      781 gacctagtgc ctaggcacaa ccagaccagc ctatataaga aagccgggtc agctgccg
//
