LOCUS Exported 869 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pFRT/TO/HIS/FLAG/HA-KIAA1967 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 869) AUTHORS Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M TITLE The mRNA-Bound Proteome and Its Global Occupancy Profile on Protein-Coding Transcripts. JOURNAL Mol Cell. 2012 Jun 8;46(5):674-90. PUBMED 22681889 REFERENCE 2 (bases 1 to 869) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..869 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 20..38 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 48..72 /label=LNCX /note="Human CMV promoter, forward primer" CDS 172..195 /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" CDS 199..222 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 223..249 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind 265..289 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg 61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg 121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat gcaccaccac 181 caccaccacc accacatgga ctacaaggac gacgatgaca agtaccctta tgacgtgccc 241 gattacgctt tgaaccagat atcaacaagt ttgtacaaaa aagcaggctc caccatggga 301 accaattcag tcgacatgtc ccagtttaag cgccagcgga tcaacccgct tccaggggga 361 cgcaacttct caggcacagc ttcaacatct cttctgggcc ctcctcctgg tttgctcact 421 cctcctgtgg ccacagaact gtcccagaat gccaggcacc ttcagggtgg ggagaaacag 481 cgggtcttca ctggtattgt taccagcttg catgactact ttggggttgt ggatgaagag 541 gtcttttttc agctaagtgt ggtgaagggc cgtctgcccc agctgggtga gaaggtgctg 601 gtgaaggctg catacaaccc aggccaggca gtgccctgga atgctgtcaa ggtgcaaacg 661 ctctccaacc agcccctact gaagtcccca gcacctcctc ttctgcatgt agcagccctg 721 ggccagaagc aagggatcct gggagctcag cctcagttga tcttccagcc tcaccggatt 781 cccccactct ttcctcagaa gcctctgagt ctcttccaaa catcccacac acttcacctg 841 agccacctga acagatttcc tgcccgggg //