LOCUS Exported 4815 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS BBa_J72110-BBa_J72094 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4815) AUTHORS Kittleson JT, DeLoache W, Cheng HY, Anderson JC TITLE Scalable plasmid transfer using engineered P1-based phagemids. JOURNAL ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30. PUBMED 23656280 REFERENCE 2 (bases 1 to 4815) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4815 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(60..938) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 965..1249 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 1172..1191 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" terminator 1501..1572 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1588..1615 /label=T7Te terminator /note="phage T7 early transcription terminator" protein_bind complement(1727..1751) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" rep_origin 1831..2376 /direction=RIGHT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind 2277..2296 /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS 2799..3458 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(2865..2884) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 3479..3573 /label=lambda t0 terminator /note="transcription terminator from phage lambda" protein_bind 3586..3619 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 3657..3761 /gene="bla" /label=AmpR promoter CDS 3762..4622 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3980..3999) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" protein_bind 4722..4746 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" terminator 4770..4813 /label=bacterial terminator /note="putative bacterial transcription terminator" ORIGIN 1 gaattcatga gatctctatg ctactccatc gagccgtcaa ttgtctgatt cgttaccaat 61 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc 121 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca 181 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg 241 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga 301 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg 361 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt 421 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt 481 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac 541 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat 601 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg 661 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac 721 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga 781 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa 841 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag 901 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc 961 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg 1021 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg 1081 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac 1141 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta 1201 gcggatctta cctgacgctt tttatcgcaa ctctctactg tttctccata cccggatctt 1261 acagtgaggc ataattatgg ctttcattcc accaaccatc gacgacgtta gacattgctc 1321 taacgcttta tctgtagacc ccgccgaaac cgacgctgcc cgcgccattg ctgaacacta 1381 ctcaaagata tccaatcagg agtaccgcat cacccaagac gacctggatg atctcactga 1441 cacaatcgaa tatctcatgg ccactaacca gccagactca caataaggat ctccaggcat 1501 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1561 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1621 aggatcctaa ctcgacgtgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg 1681 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc aattcgaccc agctttcttg 1741 tacaaagtgg ttgatcctta cagatcccgg ttatccacag aatcagggga ggccttagaa 1801 atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc 1861 ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac 1921 caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc 1981 agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg 2041 ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg 2101 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa 2161 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga 2221 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga 2281 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt 2341 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca 2401 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca 2461 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat 2521 atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg 2581 aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag 2641 cgctgatgtc cggcggtgcg catgccgtta agggattttg gtcatgaact agcttgatcg 2701 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt 2761 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat 2821 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag 2881 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg 2941 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga 3001 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg 3061 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg 3121 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg 3181 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca 3241 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg 3301 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg 3361 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat 3421 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga 3481 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc 3541 ggttgccgcc gggcgttttt tattggtgag aatccaagcc tgcagataac ttcgtatagc 3601 atacattata cgaagttatc tcgaggacgt caggtggcac ttttcgggga aatgtgcgcg 3661 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 3721 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 3781 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 3841 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 3901 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 3961 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 4021 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 4081 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 4141 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 4201 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 4261 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 4321 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 4381 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 4441 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 4501 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 4561 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 4621 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 4681 ttaaaaggat ctaggtgaag atccttgaat tgccattatc aacaagtttg tacaaaaaag 4741 caggctccga attggtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc 4801 taaggatgat ttctg //