LOCUS       Exported                 763 bp ds-DNA     linear   SYN 10-JUL-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-Wnt3-V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(7..48)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg
       61 gccctctaga tcaaccactt tgtacaagaa agctgggtcg gcgcgcccac ccttcagaat
      121 gcacttgcag gtgtgcacgt cgtagatgcg aatacactcc tggcagctga cgtagcagca
      181 ccagtggaag atgcagtggc atttttcctt ccgcttctcc gtcctcgtgt tgtggccccg
      241 gccacagcag agcagatcgc agccatcgat gccgtgggag gtgacattgc aagtccggtc
      301 ccttgtgcca aaggaacccg tctctgggtt gggctcacaa aagttggggg agttctcgta
      361 gtagaccagg tccctctccg tgggtggctt gaagagcgag tacttggccc ggagggtctc
      421 cacccagcct cgggactcac ggtgcttctc tactaccatc tccgaggcgc tgtcatactt
      481 gtccttgagg aagtcaccga tggcacggaa gtcaggctgc gcccaccagc aggtcttcac
      541 ctcacagctg cccgacagcc cgtggcactt gcatttgagg tgcatgtggt ccaggatagt
      601 cgtgcggccc gcctcgttgt tgtgcttgtt catggccgag cgcgcgtccg gcctgttctc
      661 gcgcgcatcc gcgaactccc tggacactaa cacgccgaag tcagcgtcct cgctgcagcc
      721 gccccacttc cagccttcgc caggcggccc cttatgatgc gag
//
