LOCUS Exported 4867 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMRBad-Z-CspGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4867) AUTHORS Blakeley BD, Chapman AM, McNaughton BR TITLE Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein-protein interactions in vivo. JOURNAL Mol Biosyst. 2012 Aug;8(8):2036-40. Epub 2012 Jun 12. PUBMED 22692102 REFERENCE 2 (bases 1 to 4867) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4867 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(427..445) /label=T7 Term /note="T7 terminator, reverse primer" terminator 441..488 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(1090..1905) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1813..1832 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 1897..1916 /label=pENTR-R /note="pENTR vectors, reverse primer" primer_bind complement(2366..2383) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2500..3044) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(2580..2599) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3624..4502) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 4529..4813 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 4736..4755 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" RBS 4840..4862 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" ORIGIN 1 ccatggcaag cgagcagctg gaaaagaagt tacaagccct ggagaaaaaa cttgctcagc 61 tggaatggaa aaaccaagca ttggaaaaaa aactcgcgca gacgtcgggt ggaagcggta 121 agaatggtat caaggcaaaa ttcaaaattc gccataacgt gaaagacggc agcgttcaat 181 tagcggatca ttatcaacaa aacacgccga ttggtcgcgg gcctgtactg ttacctcgca 241 accactacct gagcacccgt tctaaactga gcaaagatcc gaaagaaaaa cgcgatcaca 301 tggttctgtt agaattcgtg accgctgcag gcattaagca cggacgcgac gaacgctaca 361 agtaatgtac aactaaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg 421 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg 481 gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg 541 atggtgacca ccatttgccc catatcaccg tcggtactga tcccgtcgtc aataaaccga 601 accgctacac cctgagcatc aaactctttt atcagttgga tcatgtcggc ggtgtcgcgg 661 ccaagacggt cgagcttctt caccagaatg acatcacctt cctccacctt catcctcagc 721 aaatccagcc cttcccgatc tgttgaactg ccggatgcct tgtcggtaaa gatgcggtta 781 gcttttaccc ctgcatcttt gagcgctgag gtctgcctcg tgaagaaggt gttgctgact 841 cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga 901 gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt 961 ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc 1021 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 1081 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 1141 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 1201 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 1261 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 1321 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc 1381 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 1441 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 1501 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 1561 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa 1621 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 1681 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 1741 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga 1801 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt 1861 taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 1921 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat 1981 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga 2041 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa 2101 gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac 2161 tttctggctg gatgatgggg cgattcaggc ctggtatgag tcagcaacac cttcttcacg 2221 aggcagacct cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt 2281 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata 2341 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac 2401 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga 2461 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc 2521 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata 2581 aagataccag gcgtttcccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg 2641 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag 2701 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga 2761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc 2821 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg 2881 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc 2941 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt 3001 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta 3061 aggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3121 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 3181 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 3241 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 3301 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 3361 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 3421 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 3481 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcagat cgatgcataa 3541 tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg 3601 tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct 3661 tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag 3721 aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg 3781 gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta 3841 atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt 3901 gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc 3961 tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc 4021 agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc 4081 ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg 4141 cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg 4201 cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag 4261 tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt 4321 ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt 4381 cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc 4441 gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc 4501 atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat 4561 tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt 4621 aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc 4681 tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc 4741 atagcatttt tatccataag attagcggat cctacctgac gctttttatc gcaactctct 4801 actgtttctc catacccgtt tttttgggct agaaataatt ttgtttaact ttaagaagga 4861 gatatac //