LOCUS Exported 2430 bp ds-DNA linear SYN 12-JUL-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL3115 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2430) AUTHORS Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR TITLE Targeted gene disruption in somatic zebrafish cells using engineered TALENs. JOURNAL Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PUBMED 21822241 REFERENCE 2 (bases 1 to 2430) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2430 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 19..84 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 91..111 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 tctagagcta gcaccatgga ctacaaagac catgacggtg attataaaga tcatgacatc 61 gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt gggcattcac 121 cgcggggtac ctatggtgga cttgaggaca ctcggttatt cgcaacagca acaggagaaa 181 atcaagccta aggtcaggag caccgtcgcg caacaccacg aggcgcttgt ggggcatggc 241 ttcactcatg cgcatattgt cgcgctttca cagcaccctg cggcgcttgg gacggtggct 301 gtcaaatacc aagatatgat tgcggccctg cccgaagcca cgcacgaggc aattgtaggg 361 gtcggtaaac agtggtcggg agcgcgagca cttgaggcgc tgctgactgt ggcgggtgag 421 cttagggggc ctccgctcca gctcgacacc gggcagctgc tgaagatcgc gaagagaggg 481 ggagtaacag cggtagaggc agtgcacgcc tggcgcaatg cgctcaccgg ggcccccttg 541 aacctgaccc cagaccaggt agtcgcaatc gcgaacaata atgggggaaa gcaagccctg 601 gaaaccgtgc aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa 661 gtcgtggcca ttgcatccca cgacggtggc aaacaggctc ttgagacggt tcagagactt 721 ctcccagttc tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcc 781 aacggtggag ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa 841 gcccacggtt tgacgcctgc acaagtggtc gccatcgcct cgaatggcgg cggtaagcag 901 gcgctggaaa cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca 961 gaccaggtag tcgcaatcgc gaacaataat gggggaaagc aagccctgga aaccgtgcaa 1021 aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt 1081 gcaagcaatg ggggtggcaa acaggctctt gagacggttc agagacttct cccagttctc 1141 tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgtcgca tgacggaggg 1201 aaacaagcat tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg 1261 acgcctgcac aagtggtcgc catcgcctcc aatattggcg gtaagcaggc gctggaaaca 1321 gtacagcgcc tgctgcctgt actgtgccag gatcatggac tgaccccaga ccaggtagtc 1381 gcaatcgcgt caaacggagg gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg 1441 gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc aagcaacatc 1501 ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg tcaagcccac 1561 gggctgactc ccgatcaagt tgtagcgatt gcgtccaacg gtggagggaa acaagcattg 1621 gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa 1681 gtggtcgcca tcgcctccaa tattggcggt aagcaggcgc tggaaacagt acagcgcctg 1741 ctgcctgtac tgtgccagga tcatggactg accccagacc aggtagtcgc aatcgcgtcg 1801 aacattgggg gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa 1861 gaccacggcc ttacaccgga gcaagtcgtg gccattgcat cccacgacgg tggcaaacag 1921 gctcttgaga cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc 1981 gatcaagttg tagcgattgc gtccaacggt ggagggaaac aagcattgga gactgtccaa 2041 cggctccttc ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc 2101 gccagccatg atggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg 2161 tgccaggatc atggactgac acccgaacag gtggtcgcca ttgcttccca cgacggagga 2221 cggccagcct tggagtccat cgtagcccaa ttgtccaggc ccgatcccgc gttggctgcg 2281 ttaacgaatg accatctggt ggcgttggca tgtcttggtg gacgacccgc gctcgatgca 2341 gtcaaaaagg gtctgcctca tgctcccgca ttgatcaaaa gaaccaaccg gcggattccc 2401 gagagaactt cccatcgagt cgcgggatcc //