LOCUS       Exported                2430 bp ds-DNA     linear   SYN 05-JUL-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    TAL3154
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 5, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             19..84
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             91..111
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctagagcta gcaccatgga ctacaaagac catgacggtg attataaaga tcatgacatc
       61 gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt gggcattcac
      121 cgcggggtac ctatggtgga cttgaggaca ctcggttatt cgcaacagca acaggagaaa
      181 atcaagccta aggtcaggag caccgtcgcg caacaccacg aggcgcttgt ggggcatggc
      241 ttcactcatg cgcatattgt cgcgctttca cagcaccctg cggcgcttgg gacggtggct
      301 gtcaaatacc aagatatgat tgcggccctg cccgaagcca cgcacgaggc aattgtaggg
      361 gtcggtaaac agtggtcggg agcgcgagca cttgaggcgc tgctgactgt ggcgggtgag
      421 cttagggggc ctccgctcca gctcgacacc gggcagctgc tgaagatcgc gaagagaggg
      481 ggagtaacag cggtagaggc agtgcacgcc tggcgcaatg cgctcaccgg ggcccccttg
      541 aacctgaccc cagaccaggt agtcgcaatc gcgaacaata atgggggaaa gcaagccctg
      601 gaaaccgtgc aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa
      661 gtcgtggcca ttgcaagcaa tgggggtggc aaacaggctc ttgagacggt tcagagactt
      721 ctcccagttc tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcg
      781 catgacggag ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa
      841 gcccacggtt tgacgcctgc acaagtggtc gccatcgcct cgaatggcgg cggtaagcag
      901 gcgctggaaa cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca
      961 gaccaggtag tcgcaatcgc gtcaaacgga gggggaaagc aagccctgga aaccgtgcaa
     1021 aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt
     1081 gcatcccacg acggtggcaa acaggctctt gagacggttc agagacttct cccagttctc
     1141 tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgtccaa cggtggaggg
     1201 aaacaagcat tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg
     1261 acgcctgcac aagtggtcgc catcgccagc catgatggcg gtaagcaggc gctggaaaca
     1321 gtacagcgcc tgctgcctgt actgtgccag gatcatggac tgaccccaga ccaggtagtc
     1381 gcaatcgcgt cacatgacgg gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg
     1441 gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc aagcaatggg
     1501 ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg tcaagcccac
     1561 gggctgactc ccgatcaagt tgtagcgatt gcgtcgaaca ttggagggaa acaagcattg
     1621 gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa
     1681 gtggtcgcca tcgccagcca tgatggcggt aagcaggcgc tggaaacagt acagcgcctg
     1741 ctgcctgtac tgtgccagga tcatggactg accccagacc aggtagtcgc aatcgcgtca
     1801 aacggagggg gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa
     1861 gaccacggcc ttacaccgga gcaagtcgtg gccattgcaa ataataacgg tggcaaacag
     1921 gctcttgaga cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc
     1981 gatcaagttg tagcgattgc gtccaacggt ggagggaaac aagcattgga gactgtccaa
     2041 cggctccttc ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc
     2101 gccaacaaca acggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg
     2161 tgccaggatc atggactgac acccgaacag gtggtcgcca ttgcttctaa catcggagga
     2221 cggccagcct tggagtccat cgtagcccaa ttgtccaggc ccgatcccgc gttggctgcg
     2281 ttaacgaatg accatctggt ggcgttggca tgtcttggtg gacgacccgc gctcgatgca
     2341 gtcaaaaagg gtctgcctca tgctcccgca ttgatcaaaa gaaccaaccg gcggattccc
     2401 gagagaactt cccatcgagt cgcgggatcc
//
