LOCUS       Exported                 845 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    cytoplasmic EKAR (EGFP-mRFP)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, 
            Svoboda K.
  TITLE     A genetically encoded fluorescent sensor of ERK activity
  JOURNAL   PNAS 2008 Dec 9;105(49):19264-9
  PUBMED    19033456
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(650..671)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtacgagcgc gccgagggcc gccactccac cggcgccgag ctcaagctta tggcggacga
       61 ggagaagctg ccgcccggct gggagaagcg catgagccgc agctcaggcc gagtgtacta
      121 cttcaaccac atcactaacg ccagccagtg ggagcggccc agcggcaaca gcagcagtgg
      181 tggcaaaaac gggcaggggg agcctgccag gaagcttcga attctgcagt cgactaggga
      241 gcaagggctt gtgtctagag gcggaggtgg tggaggaggt ggaggtggtg gaggtggagg
      301 tggaggtggt ggcggtggtg gaggaggagg agctagaggc ggaggtggtg gaggaggtgg
      361 aggtggtgga ggtggaggtg gaggtggtgg cggtggtgga ggaggaggag ctagcggcgg
      421 aggtggtgga ggaggtggag gtggtggagg tggaggtgga ggtggtggcg gtggtggagg
      481 aggaggaatg gctgaccaac tgactgaaga gtggtcgacg gtaccgcgga ccggtcctga
      541 cgtaccacgt acaccagtag gaaaggcaaa gctgtcattc caattcccgc gggcccggga
      601 tcccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      661 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      721 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      781 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      841 gaagc
//
