LOCUS       Exported                 820 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3 Plk4(Sak) wt (Nigg HR9)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Habedanck R, Stierhof YD, Wilkinson CJ, Nigg EA
  TITLE     The Polo kinase Plk4 functions in centriole duplication.
  JOURNAL   Nat Cell Biol. 2005 Nov;7(11):1140-6.
  PUBMED    16244668
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             129..158
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             162..191
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             195..218
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctta ccatggagca gaagctgatc tccgaggagg
      121 acctgaacga gcagaagctg atctccgagg aggacctgaa cgagcagaag ctgatctccg
      181 aggaggacct gaacctggag gtgctgttcc agggacccgg ggtacctagg atcctgacca
      241 tggcgacctg catcggggag aagatcgagg attttaaagt tggaaatctg cttggtaaag
      301 gatcatttgc tggtgtctac agagctgagt ccattcacac tggtttggaa gttgcaatca
      361 aaatgataga taagaaagcc atgtacaaag caggaatggt acagagagtc caaaatgagg
      421 tgaaaataca ttgccaattg aaacatccct ctatcttgga gctttataac tattttgaag
      481 atagcaatta tgtgtatctg gtattagaaa tgtgccataa tggagaaatg aacaggtatc
      541 taaagaatag agtgaaaccc ttctcagaaa atgaagctcg acacttcatg caccagatca
      601 tcacagggat gttgtatctt cattctcatg gtatactaca ccgggacctc acactttcta
      661 acctcctact gactcgtaat atgaacatca agattgctga ttttgggctg gcaactcaac
      721 tgaaaatgcc acatgaaaag cactatacat tatgtggaac tcctaactac atttcaccag
      781 aaattgccac tcgaagtgca catggccttg aatctgatgt
//
