LOCUS Exported 4730 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pFA6a-GST-His3MX6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4730) AUTHORS Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR TITLE Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. JOURNAL Yeast. 1998 Jul;14(10):953-61. PUBMED 9717241 REFERENCE 2 (bases 1 to 4730) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4730 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 61..714 /codon_start=1 /product="glutathione S-transferase from Schistosoma japonicum" /label=GST /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK" primer_bind 672..694 /label=pGEX 5' /note="pGEX vectors, Glutathione-S-transferase, forward primer" terminator 756..943 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(811..835) /label=pHybLex-R /note="ADH terminator, reverse primer" promoter 1018..1361 /label=TEF promoter /note="Ashbya gossypii TEF promoter" terminator 2064..2261 /label=TEF terminator /note="Ashbya gossypii TEF terminator" primer_bind complement(2365..2384) /label=T7 /note="T7 promoter, forward primer" promoter complement(2366..2384) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2471..2488) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2642..3230) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2722..2741) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3401..4261) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4024..4043 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4262..4366) /gene="bla" /label=AmpR promoter primer_bind 4434..4452 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(4490..4512) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4612..4631 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 4712..4730 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 4712..4729 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaat 61 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 121 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa 181 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat 241 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac 301 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg 361 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt 421 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa 481 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat 541 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa 601 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca 661 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat 721 ctggttccgc gttgaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt 781 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg 841 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc 901 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata 961 acagggtaat atagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac 1021 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt 1081 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat 1141 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag 1201 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat 1261 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg 1321 ctaggataca gttctcacat cacatccgaa cataaacaac catggcagaa ccagcccaaa 1381 aaaagcaaaa acaaactgtt caggagcgca aggcgtttat ctcccgtatc actaatgaaa 1441 ctaaaattca aatcgctatt tcgctgaatg gtggttatat tcaaataaaa gattcgattc 1501 ttcctgcaaa gaaggatgac gatgtagctt cccaagctac tcagtcacag gtcatcgata 1561 ttcacacagg tgttggcttt ttggatcata tgatccatgc gttggcaaaa cactctggtt 1621 ggtctcttat tgttgaatgt atcggtgacc tgcacattga cgatcaccat actaccgaag 1681 attgcggtat cgcattaggg caagcgttca aagaagcaat gggtgctgtc cgtggtgtaa 1741 aaagattcgg tactgggttc gcaccattgg atgaggcgct atcacgtgcc gtagtcgatt 1801 tatctaatag accatttgct gtaatcgacc ttggattgaa gagagagatg attggtgatt 1861 tatccactga aatgattcca cactttttgg aaagtttcgc ggaggcggcc agaattactt 1921 tgcatgttga ttgtctgaga ggtttcaacg atcaccacag aagtgagagt gcgttcaagg 1981 ctttggctgt tgccataaga gaagctattt ctagcaatgg caccaatgac gttccctcaa 2041 ccaaaggtgt tttgatgtga agtactgaca ataaaaagat tcttgttttc aagaacttgt 2101 catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt 2161 atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa 2221 tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac 2281 gccgccatcc agtttaaacg agctcgaatt catcgatgat atcagatcca ctagtggcct 2341 atgcggccgc ggatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg 2401 ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 2461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 2521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 2581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 2641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 2701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 2761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 2821 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 2881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 2941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 3001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 3061 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 3121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 3181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 3241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 3301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 3361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 3421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 3481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 3541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 3601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 3661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 3721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 3781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 3841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 3901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 3961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 4021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 4081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 4141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 4201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 4261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 4321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 4381 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 4441 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 4501 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 4561 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 4621 agcagattgt actgagagtg caccatatgg acatattgtc gttagaacgc ggctacaatt 4681 aatacataac cttatgtatc atacacatac gatttaggtg acactataga //