LOCUS       Exported                 895 bp ds-DNA     linear   SYN 31-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    TAL-BBL1-ID43
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        141..245
                     /gene="bla"
                     /label=AmpR promoter
     promoter        247..604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(270..290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      455..590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     517..536
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(693..712)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc gaatgcaggt
       61 tgtcgcctgc agtccggatc ctggctgcag ccaggcacac gggctgcatt ccgtcaggtg
      121 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      181 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      241 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
      301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      361 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
      541 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      601 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
      661 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      721 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
      781 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
      841 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actga
//
