LOCUS       Exported                 855 bp ds-DNA     linear   SYN 03-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    7TFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      33..42
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(98..129)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    351..468
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(735..779)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgccgggcct ttctttatgt ttttggcgtc ttccatggtg gctttaccaa cagtaccgga
       61 atgccaagct ttttaccaac agtaccggaa tgccaagctg gaattcgagc ttccattata
      121 taccctctag agtctagatc tcgagcccgg gctagcacgc gggcgcgccc ctttgatctt
      181 accccctttg atcttacccc ctttgatctt accccctttg atctcgcgcc cctttgatct
      241 tacccccttt gatcttaccc cctttgatct cgcgtaagag ctcggtaccc tgcaggcatg
      301 caagcttcag ctgctcgagc ccgggctagg aggctagtct cgtgatcgat aaaattttga
      361 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
      421 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaagt taaccgatac
      481 cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct
      541 tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat
      601 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
      661 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
      721 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
      781 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
      841 tctctgtccc actcc
//
