LOCUS Exported 878 bp ds-DNA linear SYN 03-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL-1TC2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 878) AUTHORS Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V TITLE A ligation-independent cloning technique for high-throughput assembly of transcription activator-like effector genes. JOURNAL Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460. PUBMED 23242165 REFERENCE 2 (bases 1 to 878) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..878 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(307..323) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(307..323) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(320..342) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 331..347 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(355..385) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 400..421 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(538..555) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(789..808) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcgagctcgg tacctcgcga atgcatctag atccaaatga atgcagacac ccgaccaagt 61 tgtggccatt gccagcaacg gtggagggaa acaagcattg gagactgtcc aacggctcct 121 tcccgtgttg tgtcaagccc acggtttgac ccctgcacaa gtggtcgcca tcgcctccca 181 tgacggcggt aagcaggccc tggaaacagt gcaacggttg ctccctgtct tgtgtctgca 241 ttcccaaata tcggatcccg ggcccgtcga ctgcagaggc ctgcatgcaa gcttggcgta 301 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 361 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 421 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 481 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 841 gaccctgccg cttaccggat acctgtccgc ctttctcc //