LOCUS Exported 929 bp ds-DNA linear SYN 19-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL-3GG4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 929) AUTHORS Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V TITLE A ligation-independent cloning technique for high-throughput assembly of transcription activator-like effector genes. JOURNAL Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460. PUBMED 23242165 REFERENCE 2 (bases 1 to 929) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..929 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(358..374) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(358..374) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(371..393) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 382..398 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(406..436) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 451..472 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(589..606) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(840..859) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcgagctcgg tacctcgcga atgcatctag atatttggga atgcaggcga caacctggtc 61 tggggtcagt ccatgatcct ggcacagcac aggcagcagg cgctgcactg tttccagggc 121 ctgcttaccg cccttgttgg aggcgatggc gaccacttgt gcaggggtca aaccgtgggc 181 ttgacacaac acgggaagga gccgttggac agtctccaat gcttgtttcc ctcccttgtt 241 gctggcaatg gccacaactt gatcgggagt aagcccgtgt gcctggctgc attcatttgg 301 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt aatcatggtc 361 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 421 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 541 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 901 gcttaccgga tacctgtccg cctttctcc //