LOCUS Exported 953 bp ds-DNA linear SYN 06-JUL-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL-2TT3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 953) AUTHORS Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V TITLE A ligation-independent cloning technique for high-throughput assembly of transcription activator-like effector genes. JOURNAL Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460. PUBMED 23242165 REFERENCE 2 (bases 1 to 953) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 6, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..953 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(354..370) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(354..370) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(367..389) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 378..394 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(402..432) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 447..468 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(585..602) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(836..855) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcgagctcgg tacctcgcga atgcatctag atccaaatga atgcccggtt gctccctgtc 61 ttgtgtcaag atcatggact gaccccagac caggtggtcg caatcgcctc taacggtggg 121 ggaaagcaag ccctggaaac cgtgcaaagg ttgttgcccg tcctttgtca agaccacggc 181 cttacacccg agcaagtcgt ggccattgca tcaaacggtg gtggcaaaca ggctcttgag 241 actgttcaga gacttctccc agttctctgc caggcacacg ggctgcattc ccaaatatcg 301 gatcccgggc ccgtcgactg cagaggcctg catgcaagct tggcgtaatc atggtcatag 361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tag //