LOCUS Exported 882 bp ds-DNA linear SYN 19-JUL-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL-4CC1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 882) AUTHORS Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V TITLE A ligation-independent cloning technique for high-throughput assembly of transcription activator-like effector genes. JOURNAL Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460. PUBMED 23242165 REFERENCE 2 (bases 1 to 882) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..882 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(322..338) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(322..338) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(335..357) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 346..362 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(370..400) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 415..436 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(553..570) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(804..823) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcgagctcgg tacctcgcga atgcatctag atccaaatga atgcaggttg tcgccatcgc 61 ctctcatgac gggggaaagc aagccctgga aaccgtgcaa aggttgttgc ccgtcctttg 121 tcaagaccac ggccttacac ccgagcaagt cgtggccatt gcatcacatg acggtggcaa 181 acaggctctt gagactgttc agagacttct cccagttctc tgtcaagccc acggtttgac 241 acccgaccaa gggcattccc aaatatcgga tcccgggccc gtcgactgca gaggcctgca 301 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 361 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 421 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tc //