LOCUS       Exported                 926 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  3rd gen lentiviral Tetracycline repressor expression vector, CMV 
            promoter, Blast .
ACCESSION   .
VERSION     .
KEYWORDS    pLenti CMV TetR Blast (716-1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             25..642
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     primer_bind     complement(98..117)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    complement(673..697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(781..801)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cgactcacta tagggcgaat tgatatgtct agattagata aaagtaaagt gattaacagc
       61 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
      121 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
      181 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
      241 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
      301 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
      361 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
      421 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
      481 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
      541 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
      601 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgcgta cagcggatcc
      661 cgggaattct agacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc
      721 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
      781 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
      841 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
      901 ggagttgtgg cccgttgtca ggcaac
//
