LOCUS Exported 396 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION An empty gRNA expression vector, used to create a gRNA to a specific sequence.. ACCESSION . VERSION . KEYWORDS gRNA_Cloning Vector SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 396) AUTHORS Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM TITLE RNA-Guided Human Genome Engineering via Cas9. JOURNAL Science. 2013 Jan 3. PUBMED 23287722 REFERENCE 2 (bases 1 to 396) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..396 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 70..90 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 241..260 /label=LKO.1 5' /note="Human U6 promoter, forward primer" protein_bind complement(367..391) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 tgtacaaaaa agcaggcttt aaaggaacca attcagtcga ctggatccgg taccaaggtc 61 gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct 121 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg 181 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg 241 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatctta 301 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt 361 ttctagaccc agctttcttg tacaaagttg gcatta //