LOCUS Exported 5856 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral expression of human OCT3/4. ACCESSION . VERSION . KEYWORDS pMXs-hOCT3/4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5856) AUTHORS Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S TITLE Induction of pluripotent stem cells from adult human fibroblasts by defined factors. JOURNAL Cell. 2007 Nov 30. 131(5):861-72. PUBMED 18035408 REFERENCE 2 (bases 1 to 5856) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5856 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(2..21) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(674..1534) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1297..1316 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1535..1639) /gene="bla" /label=AmpR promoter LTR 1669..2262 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 2325..2682 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 2741..3157 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 3087..3109 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 3125..3141 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 3167..3541 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 3479..3495 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" LTR 5009..5478 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(5607..5624) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5778..510) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 601 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa 661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 1021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 1081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 1141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 1201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 1261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 1321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 1381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 1441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 1501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 1561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 1621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcataa tgaaagaccc 1681 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac 1741 ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg 1801 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg 1861 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg 1921 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca 1981 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa 2041 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc 2101 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta 2161 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc 2221 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat 2281 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gccagcaact 2341 tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct gcgtcggtac 2401 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttcggaaca 2461 cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg tggcccgacc 2521 tgagtccaaa aatcccgatc gttttggact ctttggtgca cccccctaat aggagggata 2581 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct 2641 ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg ttctgtgttg 2701 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc 2761 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt 2821 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt 2881 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt 2941 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc 3001 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc 3061 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc 3121 ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt 3181 atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa 3241 cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag 3301 acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg 3361 agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa 3421 aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc 3481 ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact 3541 gccggatcta gctagttaat taaggatccc agtgtggtgg tacgggaatt cgcccttcac 3601 catggcggga cacctggctt cggatttcgc cttctcgccc cctccaggtg gtggaggtga 3661 tgggccaggg gggccggagc cgggctgggt tgatcctcgg acctggctaa gcttccaagg 3721 ccctcctgga gggccaggaa tcgggccggg ggttgggcca ggctctgagg tgtgggggat 3781 tcccccatgc cccccgccgt atgagttctg tggggggatg gcgtactgtg ggccccaggt 3841 tggagtgggg ctagtgcccc aaggcggctt ggagacctct cagcctgagg gcgaagcagg 3901 agtcggggtg gagagcaact ccgatggggc ctccccggag ccctgcaccg tcacccctgg 3961 tgccgtgaag ctggagaagg agaagctgga gcaaaacccg gaggagtccc aggacatcaa 4021 agctctgcag aaagaactcg agcaatttgc caagctcctg aagcagaaga ggatcaccct 4081 gggatataca caggccgatg tggggctcac cctgggggtt ctatttggga aggtattcag 4141 ccaaacgacc atctgccgct ttgaggctct gcagcttagc ttcaagaaca tgtgtaagct 4201 gcggcccttg ctgcagaagt gggtggagga agctgacaac aatgaaaatc ttcaggagat 4261 atgcaaagca gaaaccctcg tgcaggcccg aaagagaaag cgaaccagta tcgagaaccg 4321 agtgagaggc aacctggaga atttgttcct gcagtgcccg aaacccacac tgcagcagat 4381 cagccacatc gcccagcagc ttgggctcga gaaggatgtg gtccgagtgt ggttctgtaa 4441 ccggcgccag aagggcaagc gatcaagcag cgactatgca caacgagagg attttgaggc 4501 tgctgggtct cctttctcag ggggaccagt gtcctttcct ctggccccag ggccccattt 4561 tggtacccca ggctatggga gccctcactt cactgcactg tactcctcgg tccctttccc 4621 tgagggggaa gcctttcccc ctgtctctgt caccactctg ggctctccca tgcattcaaa 4681 ctgaggtaag ggcgaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg 4741 tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat 4801 tatgtactat ttaaactcga gcggccgcca gcacagtggt cgacgataaa ataaaagatt 4861 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta 4921 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc 4981 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta 5041 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac 5101 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag 5161 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag 5221 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt 5281 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc 5341 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt 5401 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc 5461 gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt tttcttaagt 5521 atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt 5581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 5641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 5701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 5761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 5821 cgctcaagtc agaggtggcg aaacccgaca ggacta //