LOCUS       Exported               10997 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  This plasmid is used to reconstitute the complete Type II CRISPR 
            system from S. pyogenes and contains the host factor RNaseIII and 
            tracrRNA..
ACCESSION   .
VERSION     .
KEYWORDS    pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 10997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10997
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2397..2637
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     2397..2417
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2568..2587
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2646..2724
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     misc_feature    2781..2898
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3023..4200
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3254..4191
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4148..4168
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4909..5613
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5051..5069)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5319..5338
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5614..5661
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    5689..6277
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5742..5762)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6160..6171)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6280..6296)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6281..6297)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6802..6982
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7008..7025)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7014..7238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7284..7712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7371..7390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7581..7602
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7721..7741)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7726..8055
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7906..8041
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7968..7987
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8103..8150
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8169..8543
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8673..8794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8710..8729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8764..8783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8843..8859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8843..8859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8856..8878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8867..8883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8891..8921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8936..8957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9074..9091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9245..9833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9325..9344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10004..10864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10627..10646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10865..10969)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgagg
     2401 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     2461 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     2521 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     2581 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     2641 acaccggaac cattcaaaac agcatagcaa gttaaaataa ggctagtccg ttatcaactt
     2701 gaaaaagtgg caccgagtcg gtgctttttt tgctagcgcg tgcgccaatt ctgcagacaa
     2761 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2821 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2881 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     2941 aatgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     3001 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3061 cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg
     3121 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     3181 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     3241 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     3301 tggcccttgc gtgccttgaa ttacttccac tggctgcagt acgtgattct tgatcccgag
     3361 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     3421 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     3481 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     3541 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     3601 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     3661 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     3721 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     3781 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     3841 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     3901 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     3961 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     4021 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     4081 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     4141 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     4201 cgtacggcca ccatgaagca gctggaggag ttactttcta cctctttcga catccagttt
     4261 aatgacctga ccctgctgga aaccgccttc actcacacct cctacgcgaa tgagcaccgc
     4321 ctactgaatg tgagccacaa cgagcgcctg gagtttctgg gggatgctgt cttacagctg
     4381 atcatctctg aatatctgtt tgccaaatac cctaagaaaa ccgaagggga catgtcaaag
     4441 ctgcgctcca tgatagtcag ggaagagagc ctggcgggct ttagtcgttt ttgctcattc
     4501 gacgcttata tcaagctggg aaaaggcgaa gagaagtccg gcggcaggag gcgcgataca
     4561 attctgggcg atctctttga agcgtttctg ggcgcacttc tactggacaa agggatcgac
     4621 gcagtccgcc gctttctgaa acaagtgatg atccctcagg tcgaaaaggg aaacttcgag
     4681 agagtgaagg actataaaac atgtttgcag gaatttctcc agaccaaggg agatgtagca
     4741 atagattatc aggtaataag tgagaaagga ccagctcacg ccaaacaatt cgaagttagc
     4801 atcgttgtta atggcgcagt gttgtcgaag ggcttgggta aatcaaaaaa actggccgag
     4861 caggacgctg ctaaaaacgc cctcgctcag ctcagcgagg taggatccgt gagcaagggc
     4921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     4981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     5041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     5101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     5161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     5221 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     5281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     5341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     5401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     5461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     5521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     5581 cactccaccg gcggcatgga cgagctgtac aagaagcgtc ctgctgctac taagaaagct
     5641 ggtcaagcta agaaaaagaa ataagaattc gatatcaagc ttatcgataa tcaacctctg
     5701 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5761 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5821 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5881 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5941 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6001 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6061 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6121 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6181 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6241 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     6301 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     6361 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     6421 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6481 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     6541 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     6601 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     6661 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     6721 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     6781 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6841 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6901 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6961 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     7021 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7081 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7141 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7201 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7261 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7441 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7501 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7561 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7621 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7681 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7801 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7861 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7921 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7981 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8041 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     8101 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     8161 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     8221 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     8281 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     8341 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     8401 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     8461 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     8521 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     8581 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     8641 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     8701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8761 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     8821 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8881 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8941 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9001 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     9061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    10321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10621 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10981 gaaaagtgcc acctgac
//
