LOCUS Exported 976 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Expresses human codon optimized Cas9 nuclease for genome engineering. ACCESSION . VERSION . KEYWORDS hCas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 976) AUTHORS Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM TITLE RNA-Guided Human Genome Engineering via Cas9. JOURNAL Science. 2013 Jan 3. PUBMED 23287722 REFERENCE 2 (bases 1 to 976) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..976 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(31..51) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 ttactaaccg gtagggatcg aaccctttca caccttcctc ttcttcttgg ggtcagccct 61 gctgtctcca ccgagctgag agaggtcgat tcttgtttca tagagccccg taattgactg 121 atgaatcagt gtggcgtcca ggacctcctt tgtagaggtg taccgctttc tgtctatggt 181 ggtgtcgaag tacttgaagg ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat 241 aatgttttct gcctgctccc tgatgggctt atccctgtgc ttattgtaag cagaaagcac 301 cttatcgagg ttagcgtcgg cgaggatcac tcttttggag aattcgctta tttgctcgat 361 gatctcatca aggtagtgtt tgtgttgttc cacgaacagc tgcttctgct cattatcttc 421 gggagaccct ttgagctttt catagtggct ggccagatac aagaaattaa cgtatttaga 481 gggcagtgcc agctcgttac ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg 541 gccgttttca agctcaaaga gagagtactt gggaagctta atgatgaggt cttttttgac 601 ctctttatat cctttcgcct cgagaaagtc gatggggttt ttttcgaagc ttgatcgctc 661 catgattgtg atgcccagca gttccttgac gcttttgagt tttttagact tccctttctc 721 cactttggcc acaaccagta cactgtaagc gactgtagga gaatcgaatc cgccgtattt 781 cttggggtcc caatcttttt tgcgtgcgat cagcttgtcg ctgttccttt tcgggaggat 841 actttccttg gagaagcctc cggtctgtac ttcggtcttt ttaacgatgt tcacctgcgg 901 catggacagg accttccgga ctgtcgcgaa atccctaccc ttgtcccaca cgatttctcc 961 tgtttctccg tttgtt //