LOCUS       Exported                 780 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  This plasmid is used to reconstitute the complete Type II CRISPR 
            system from S. pyogenes and contains the host factor RNaseIII and 
            tracrRNA..
ACCESSION   .
VERSION     .
KEYWORDS    pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
       61 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcacgga
      121 tcctacctcg ctgagctgag cgagggcgtt tttagcagcg tcctgctcgg ccagtttttt
      181 tgatttaccc aagcccttcg acaacactgc gccattaaca acgatgctaa cttcgaattg
      241 tttggcgtga gctggtcctt tctcacttat tacctgataa tctattgcta catctccctt
      301 ggtctggaga aattcctgca aacatgtttt atagtccttc actctctcga agtttccctt
      361 ttcgacctga gggatcatca cttgtttcag aaagcggcgg actgcgtcga tccctttgtc
      421 cagtagaagt gcgcccagaa acgcttcaaa gagatcgccc agaattgtat cgcgcctcct
      481 gccgccggac ttctcttcgc cttttcccag cttgatataa gcgtcgaatg agcaaaaacg
      541 actaaagccc gccaggctct cttccctgac tatcatggag cgcagctttg acatgtcccc
      601 ttcggttttc ttagggtatt tggcaaacag atattcagag atgatcagct gtaagacagc
      661 atcccccaga aactccaggc gctcgttgtg gctcacattc agtaggcggt gctcattcgc
      721 gtaggaggtg tgagtgaagg cggtttccag cagggtcagg tcattaaact ggatgtcgaa
//
