LOCUS Exported 2328 bp ds-DNA linear SYN 03-SEP-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL3277 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2328) AUTHORS Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR TITLE Targeted gene disruption in somatic zebrafish cells using engineered TALENs. JOURNAL Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PUBMED 21822241 REFERENCE 2 (bases 1 to 2328) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2328 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 19..84 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 91..111 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 tctagagcta gcaccatgga ctacaaagac catgacggtg attataaaga tcatgacatc 61 gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt gggcattcac 121 cgcggggtac ctatggtgga cttgaggaca ctcggttatt cgcaacagca acaggagaaa 181 atcaagccta aggtcaggag caccgtcgcg caacaccacg aggcgcttgt ggggcatggc 241 ttcactcatg cgcatattgt cgcgctttca cagcaccctg cggcgcttgg gacggtggct 301 gtcaaatacc aagatatgat tgcggccctg cccgaagcca cgcacgaggc aattgtaggg 361 gtcggtaaac agtggtcggg agcgcgagca cttgaggcgc tgctgactgt ggcgggtgag 421 cttagggggc ctccgctcca gctcgacacc gggcagctgc tgaagatcgc gaagagaggg 481 ggagtaacag cggtagaggc agtgcacgcc tggcgcaatg cgctcaccgg ggcccccttg 541 aacctgaccc cagaccaggt agtcgcaatc gcgtcacatg acgggggaaa gcaagccctg 601 gaaaccgtgc aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa 661 gtcgtggcca ttgcaagcaa tgggggtggc aaacaggctc ttgagacggt tcagagactt 721 ctcccagttc tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcg 781 catgacggag ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa 841 gcccacggtt tgacgcctgc acaagtggtc gccatcgcct cgaatggcgg cggtaagcag 901 gcgctggaaa cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca 961 gaccaggtag tcgcaatcgc gaacaataat gggggaaagc aagccctgga aaccgtgcaa 1021 aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt 1081 gcaagcaatg ggggtggcaa acaggctctt gagacggttc agagacttct cccagttctc 1141 tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgtcgca tgacggaggg 1201 aaacaagcat tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg 1261 acgcctgcac aagtggtcgc catcgcctcg aatggcggcg gtaagcaggc gctggaaaca 1321 gtacagcgcc tgctgcctgt actgtgccag gatcatggac tgaccccaga ccaggtagtc 1381 gcaatcgcgt cacatgacgg gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg 1441 gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc aagcaatggg 1501 ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg tcaagcccac 1561 gggctgactc ccgatcaagt tgtagcgatt gcgaataaca atggagggaa acaagcattg 1621 gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa 1681 gtggtcgcca tcgcctccaa tattggcggt aagcaggcgc tggaaacagt acagcgcctg 1741 ctgcctgtac tgtgccagga tcatggactg accccagacc aggtagtcgc aatcgcgaac 1801 aataatgggg gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa 1861 gaccacggcc ttacaccgga gcaagtcgtg gccattgcaa gcaatggggg tggcaaacag 1921 gctcttgaga cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc 1981 gatcaagttg tagcgattgc gtcgcatgac ggagggaaac aagcattgga gactgtccaa 2041 cggctccttc ccgtgttgtg tcaagcccac ggtctgacac ccgaacaggt ggtcgccatt 2101 gctaataata acggaggacg gccagccttg gagtccatcg tagcccaatt gtccaggccc 2161 gatcccgcgt tggctgcgtt aacgaatgac catctggtgg cgttggcatg tcttggtgga 2221 cgacccgcgc tcgatgcagt caaaaagggt ctgcctcatg ctcccgcatt gatcaaaaga 2281 accaaccggc ggattcccga gagaacttcc catcgagtcg cgggatcc //