LOCUS       Exported                 379 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMJ920
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Jinek M, East A, Cheng A, Lin S, Ma E, Doudna J
  TITLE     RNA-programmed genome editing in human cells.
  JOURNAL   elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29.
  PUBMED    23386978
REFERENCE   2  (bases 1 to 379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(59..79)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(86..106)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(125..151)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctcctcgcc cttgctcacg gatccgctgc tgccgttgct cccactacca atgccgtcca
       61 ctttccgctt tttcttagga tcttccacct tgcgtttttt cttgggggag ccgctgccca
      121 ggctggcata atcgggcacg tcatagggat aggcgtcgcc tcccagctga gacaggtcga
      181 tccgtgtctc gtacaggccg gtgatgctct ggtggatcag ggtggcgtcc agcacctctt
      241 tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa gtacttgaag gcggcagggg
      301 ctcccagatt ggtcagggta aacaggtgga tgatattctc ggcctgctct ctgataggct
      361 tgtctctgtg cttgttgta
//
