LOCUS Exported 859 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).. ACCESSION . VERSION . KEYWORDS pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 859) AUTHORS Hermann M, Cermak T, Voytas DF, Pelczar P TITLE Mouse genome engineering using designer nucleases. JOURNAL J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930. PUBMED 24747757 REFERENCE 2 (bases 1 to 859) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..859 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 769..790 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 805..835 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 843..859 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 tgatctcgcc gttgttgaac ttgcgccgca cctcctccag tgtcagggtg ccggctttga 61 tcatctcgcc gccgatcagc agctcctcca cgctcagcac ggcgccattg cagttggtga 121 tgtggttcag cctggtcagc tgggccttgt agttgccctt gaagtggccg ctcacgaaca 181 ggaacttgaa ctcggtcacg ctgctagggt acaccttcca ccactcgttg gggttgaggt 241 gcttatcccg tgtctggttc tcctccacgt atctctccat ctcgtcggcc tggccgatag 301 gcagattgta gccgccgctg taggcctttg tgtccacgat cacgccgtaa tcgatggggc 361 tgcccactgt atagatggcg ccgtcaggct ttctgcttcc gcccaggtgc tctcccctgt 421 agccgtacac cttcatgaag aactccatca ccttcatctc caggatgcgg tcctgggtgg 481 ggttcctggc gatctcgatc agctcgatgt actcgtgggg cacgtacttc agcttgtgcc 541 gcagctcgga cttcttctcc tccagctcgc tcttcaccag ctgggatccc gcaacgcgat 601 gggacgtgcg ttcgccaata cggcgattga ctcttctgat caattccggc gcgtgcggca 661 atcccttttt cactgcatcc atggcaggac gtccgccgag gcaggccaag gcgacgaggt 721 ggtcgttgga gacgaccggt ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 781 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 841 aattgtgagc ggataacaa //