LOCUS       Exported                 768 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Non-integrating (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    pCE-hSK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(230..295)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 cgccgctctc caggtctgtg gccacggtcg ccgccgccag gtcatagggg cggccgggaa
       61 gcactggggg aagtcgcttc atgtgggaga gctcctcccg ccagcggtta ttcggggcac
      121 ctgcttgacg cagtgtcttc tcccttcccg ccgggccaga cgcgaacgtg gagaaagatg
      181 ggagcagcgc gtcactgaca gccatggtga agggcgaatt gttcatgagt ggacctggat
      241 tgctttctac atccccagcc agtttgagta aatcaaagtt aagagtttgt ttgacaggag
      301 cgacaatttt gagatccccc atgtgtgaga ggggcagtgt gccgttaatg gccgtgccgg
      361 gcaccgggcc gctctggtag tgctgggaca tgtgaagtct gctgggggcg gcgggttccg
      421 gcacctcggc gccggggaga tacatgctga tcatgtcccg gaggtccccg gcctggcagg
      481 gcgccctgga gtgggaggaa gaggtaacca caggggggct ggagctggcc tcggacttga
      541 ccaccgaacc catggagcca agagccatgc caggggtgcc ctgctgcgag taggacatgc
      601 tgtaggtggg cgagccgttc atgtaggtct gcgagctggt catggagttg tactgcaggg
      661 cgctcacgtc gtagcggtgc atgggctgca tctgcgctgc gccgtgcgca ttgaggcccg
      721 ggtgctgcgg gtagcccagc tggtcctgca tcatgctgta gctgccgt
//
