LOCUS       Exported                 863 bp ds-DNA     linear   SYN 27-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA/Myc DeltaN TCF4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Korinek V, Barker N, Morin PJ, van Wichen D, de Weger R, Kinzler KW,
            Vogelstein B, Clevers H
  TITLE     Constitutive transcriptional activation by a beta-catenin-Tcf 
            complex in APC-/- colon carcinoma.
  JOURNAL   Science. 1997 Mar 21. 275(5307):1784-7.
  PUBMED    9065401
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           94..136
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      158..167
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             167..196
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgcttgttc tttttgcaga agctcagaat
      121 aaacgctcaa ctttggcaga tctgatctag aactagtgcc gccatggagc agaagctgat
      181 cagcgaggag gacctgcagc ccggggatcc cgaaaactcc tcggcagaga gggatttagc
      241 tgatgtcaaa tcgtctctag tcaatgaatc agaaacgaat caaaacagct cctccgattc
      301 cgaggcggaa agacggcctc cgcctcgctc cgaaagtttc cgagacaaat cccgggaaag
      361 tttggaagaa gcggccaaga ggcaagatgg agggctcttt aaggggccac cgtatcccgg
      421 ctaccccttc atcatgatcc ccgacctgac gagcccctac ctccccaacg gatcgctctc
      481 gcccaccgcc cgaacctatc tccagatgaa atggccactg cttgatgtcc aggcagggag
      541 cctccagagt agacaagccc tcaaggatgc ccggtcccca tcaccggcac acattgtctc
      601 taacaaagtg ccagtggtgc agcaccctca ccatgtccac cccctcacgc ctcttatcac
      661 gtacagcaat gaacacttca cgccgggaaa cccacctcca cacttaccag ccgacgtaga
      721 ccccaaaaca ggaatcccac ggcctccgca ccctccagat atatccccgt attacccact
      781 atcgcctggc accgtaggac aaatccccca tccgctagga tggttagtac cacagcaagg
      841 tcaaccagtg tacccaatca cga
//
