LOCUS       Exported               12352 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    tetO-ALN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12352)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 12352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12352
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2610..2880
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2950
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2947..2971
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3050..3069
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3050..3068
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3100..3124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3845..3901
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     RBS             4898..4906
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5027..5080
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6876..6900)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6926..6967
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6977..6994
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    7044..7632
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7097..7117)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7515..7526)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7635..7651)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7636..7652)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8157..8337
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8363..8380)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8369..8593
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8639..9067
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8726..8745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8936..8957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9076..9096)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9081..9410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9261..9396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9323..9342
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9458..9505
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9524..9898
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10028..10149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10065..10084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10119..10138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10198..10214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10198..10214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10211..10233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10222..10238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10246..10276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10291..10312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10429..10446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10600..11188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10680..10699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11359..12219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11982..12001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12220..12324)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
     2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattgt aatacgactc
     3061 actataggga gacccaagct ggctaggtaa gcttgatcaa caagtttgta caaaaaagca
     3121 ggctaccatg gaaagctctg ccaagatgga gagcggcggc gccggccagc agccccagcc
     3181 gcagccccag cagcccttcc tgccgcccgc agcctgtttc tttgccacgg ccgcagccgc
     3241 ggcggccgca gccgccgcag cggcagcgca gagcgcgcag cagcagcagc agcagcagca
     3301 gcagcagcag caggcgccgc agctgagacc ggcggccgac ggccagccct cagggggcgg
     3361 tcacaagtca gcgcccaagc aagtcaagcg acagcgctcg tcttcgcccg aactgatgcg
     3421 ctgcaaacgc cggctcaact tcagcggctt tggctacagc ctgccgcagc agcagccggc
     3481 cgccgtggcg cgccgcaacg agcgcgagcg caaccgcgtc aagttggtca acctgggctt
     3541 tgccaccctt cgggagcacg tccccaacgg cgcggccaac aagaagatga gtaaggtgga
     3601 gacactgcgc tcggcggtcg agtacatccg cgcgctgcag cagctgctgg acgagcatga
     3661 cgcggtgagc gccgccttcc aggcaggcgt cctgtcgccc accatctccc ccaactactc
     3721 caacgacttg aactccatgg ccggctcgcc ggtctcatcc tactcgtcgg acgagggctc
     3781 ttacgacccg ctcagccccg aggagcagga gcttctcgac ttcaccaact ggttcggatc
     3841 cggtgccacg aacttctctc tgttaaagca agcaggagat gttgaagaaa accccgggcc
     3901 tatgttggac ggcatcaaga tggaggagca cgccctgcgc cccgggcccg ccactctggg
     3961 ggtgctgctg ggctccgact gcccgcatcc cgccgtctgc gagggctgcc agcggcccat
     4021 ctccgaccgc ttcctgatgc gagtcaacga gtcgtcctgg cacgaggagt gtttgcagtg
     4081 cgcggcgtgt cagcaagccc tcaccaccag ctgctacttc cgggatcgga aactgtactg
     4141 caaacaagac taccaacagc tcttcgcggc caagtgcagc ggctgcatgg agaagatcgc
     4201 ccccaccgag ttcgtgatgc gggcgctgga gtgcgtgtac cacctgggct gcttctgctg
     4261 ctgcgtgtgt gaacggcagc tacgcaaggg cgacgaattc gtgctcaagg agggccagct
     4321 gctgtgcaag ggtgactacg agaaggagaa ggacctgctc agctccgtga gccccgacga
     4381 gtccgactcc gtgaagagcg aggatgaaga tggggacatg aagccggcca aggggcaggg
     4441 cagtcagagc aagggcagcg gggatgacgg gaaggacccg cggaggccca agcgaccccg
     4501 gaccatcctc accacgcagc agcgaagagc cttcaaggcc tccttcgagg tctcgtcgaa
     4561 gccttgccga aaggtccgag agacactggc agctgagacg ggcctcagtg tgcgcgtggt
     4621 ccaggtctgg tttcagaacc aaagagcaaa gatgaagaag ctggcgcggc ggcaccagca
     4681 gcagcaggag cagcagaact cccagcggct gggccaggag gtcctgtcca gccgcatgga
     4741 gggcatgatg gcttcctaca cgccgctggc cccaccacag cagcagatcg tggccatgga
     4801 acagagcccc tacggcagca gcgacccctt ccagcagggc ctcacgccgc cccaaatgcc
     4861 agggaacgac tccatcttcc atgacatcga cagcgatacc tccttaacca gcctcagcga
     4921 ctgcttcctc ggctcctcag acgtgggctc cctgcaggcc cgcgtgggga accccatcga
     4981 ccggctctac tccatgcaga gttcctactt cgcctccgga tccggagagg gcagaggaag
     5041 tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgccttgtg ttcaggcgca
     5101 gtatgggtcc tcgcctcaag gagccagccc cgcttctcag agctacagtt accactcttc
     5161 gggagaatac agctccgatt tcttaactcc agagtttgtc aagtttagca tggacctcac
     5221 caacactgaa atcactgcca ccacttctct ccccagcttc agtaccttta tggacaacta
     5281 cagcacaggc tacgacgtca agccaccttg cttgtaccaa atgcccctgt ccggacagca
     5341 gtcctccatt aaggtagaag acattcagat gcacaactac cagcaacaca gccacctgcc
     5401 cccccagtct gaggagatga tgccgcactc cgggtcggtt tactacaagc cctcctcgcc
     5461 cccgacgccc accaccccgg gcttccaggt gcagcacagc cccatgtggg acgacccggg
     5521 atctctccac aacttccacc agaactacgt ggccactacg cacatgatcg agcagaggaa
     5581 aacgccagtc tcccgcctct ccctcttctc ctttaagcaa tcgccccctg gcaccccggt
     5641 gtctagttgc cagatgcgct tcgacgggcc cctgcacgtc cccatgaacc cggagcccgc
     5701 cggcagccac cacgtggtgg acgggcagac cttcgctgtg cccaacccca ttcgcaagcc
     5761 cgcgtccatg ggcttcccgg gcctgcagat cggccacgcg tctcagctgc tcgacacgca
     5821 ggtgccctca ccgccgtcgc ggggctcccc ctccaacgag gggctgtgcg ctgtgtgtgg
     5881 ggacaacgcg gcctgccaac actacggcgt gcgcacctgt gagggctgca aaggcttctt
     5941 taagcgcaca gtgcaaaaaa atgcaaaata cgtgtgttta gcaaataaaa actgcccagt
     6001 ggacaagcgt cgccggaatc gctgtcagta ctgccgattt cagaagtgcc tggctgttgg
     6061 gatggtcaaa gaagtggttc gcacagacag tttaaaaggc cggagaggtc gtttgccctc
     6121 gaaaccgaag agcccacagg agccctctcc cccttcgccc ccggtgagtc tgatcagtgc
     6181 cctcgtcagg gcccatgtcg actccaaccc ggctatgacc agcctggact attccaggtt
     6241 ccaggcgaac cctgactatc aaatgagtgg agatgacacc cagcatatcc agcaattcta
     6301 tgatctcctg actggctcca tggagatcat ccggggctgg gcagagaaga tccctggctt
     6361 cgcagacctg cccaaagccg accaagacct gctttttgaa tcagctttct tagaactgtt
     6421 tgtccttcga ttagcataca ggtccaaccc agtggagggt aaactcatct tttgcaatgg
     6481 ggtggtcttg cacaggttgc aatgcgttcg tggctttggg gaatggattg attccattgt
     6541 tgaattctcc tccaacttgc agaatatgaa catcgacatt tctgccttct cctgcattgc
     6601 tgccctggct atggtcacag agagacacgg gctcaaggaa cccaagagag tggaagaact
     6661 gcaaaacaag attgtaaatt gtctcaaaga ccacgtgact ttcaacaatg gggggttgaa
     6721 ccgccccaat tatttgtcca aactgttggg gaagctccca gaacttcgta ccctttgcac
     6781 acaggggcta cagcgcattt tctacctgaa attggaagac ttggtgccac cgccagcaat
     6841 aattgacaaa cttttcctgg acactttacc tttctaccca gctttcttgt acaaagtggt
     6901 tgatctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     6961 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     7021 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     7081 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7141 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7201 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7261 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7321 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7381 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7441 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7501 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7561 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7621 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     7681 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     7741 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     7801 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7861 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     7921 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     7981 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     8041 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     8101 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     8161 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8221 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8281 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     8341 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8401 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8461 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8521 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8581 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     8641 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8701 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8761 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8821 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8881 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8941 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9001 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9061 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     9361 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     9421 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     9481 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     9541 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     9601 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     9661 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     9721 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     9781 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     9841 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     9901 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     9961 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    10021 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10141 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    10201 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    10261 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    10321 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    10381 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    10441 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10501 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    10561 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    10621 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    10681 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    10741 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    10801 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    10861 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    10921 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    10981 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    11041 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    11101 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    11161 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    11221 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    11281 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    11341 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    11401 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    11461 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    11521 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    11581 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    11641 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    11701 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    11761 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    11821 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    11881 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    11941 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    12001 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    12061 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12121 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12181 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    12241 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    12301 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
