LOCUS Exported 10656 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS CLPIT Cry2PHR-mCh-RhoA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10656) AUTHORS Bugaj LJ, Choksi AT, Mesuda CK, Kane RS, Schaffer DV TITLE Optogenetic protein clustering and signaling activation in mammalian cells. JOURNAL Nat Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360. PUBMED 23377377 REFERENCE 2 (bases 1 to 10656) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10656 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(23..43) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" enhancer 151..454 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 459..662 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 613..633 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 901..1258 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1323..1739 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1669..1691 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1707..1723 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind 2061..2077 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" CDS 2137..2736 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind complement(2137..2156) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 2633..2653 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 2816..3400 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2982..2999) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3209..3228 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3401..4411 /codon_start=1 /product="tetracycline-controlled transactivator, comprising a fusion of the tetracycline repressor TetR with the C-terminal activation domain of herpes simplex virus VP16" /label=tTA /note="In the Tet-Off(R) system, tTA binds to the Tet-responsive element and stimulates transcription only in the absence of tetracycline or doxycycline." /translation="MGSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGST IEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAH ADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG" primer_bind complement(3477..3496) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" protein_bind 4470..4488 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4512..4530 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4554..4572 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4596..4614 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4638..4656 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4680..4698 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" promoter 4770..4807 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4804..4828 /label=LNCX /note="Human CMV promoter, forward primer" CDS 4894..6387 /codon_start=1 /product="N-terminal photolyase homology region of Arabidopsis thaliana cryptochrome 2" /label=CRY2PHR /note="binds to CIB1 upon exposure to blue light (Kennedy et al., 2010)" /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA" regulatory 6406..6415 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6412..7119 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(6557..6575) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 6825..6844 /label=mCherry-F /note="mCherry, forward primer" CDS 7150..7728 /codon_start=1 /product="human Rho family small GTPase RhoA" /label=RhoA /translation="AAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADI EVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKH FCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTK DGVREVFEMATRAALQARRGKKKSGCLVL" primer_bind complement(7771..7787) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7771..7787) /label=pBluescriptSK /note="For pBluescript vector" LTR 8068..8537 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(8730..8747) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8901..9489) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8981..9000) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9660..10520) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10283..10302 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10521..10625) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatccgct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 61 tccccagcag gcagaagtat gcaaagtgac tagttattaa tagtaatcaa ttacggggtc 121 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 181 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 241 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 301 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 361 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 421 gtacatctac gtattagtca tcgctattac catgcatggt gatgcggttt tggcagtaca 481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 661 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg 721 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 781 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg 841 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt 901 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat 961 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac 1021 tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact tcgggggccg 1081 tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc 1141 ccttagagga gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc 1201 cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca 1261 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aatatgggcc 1321 cgggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc 1381 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg 1441 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca 1501 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc 1561 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg 1621 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc 1681 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc 1741 ccatatggcc atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc 1801 ctgacatgac aagagttact aacagcccct ctctccaagc tcacttacag gctctctact 1861 tagtccagca cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac 1921 cggtggtacc tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta 1981 agaacctaga acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc 2041 tcaaagtaga cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg 2101 ggggtggacc atcctctagc gtttctgaag cttaccatga ccgagtacaa gcccacggtg 2161 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc 2221 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 2281 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 2341 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 2401 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 2461 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 2521 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 2581 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 2641 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc gaaggaccgc 2701 gcgacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc 2761 ccgaccgaaa ggagcgcacg accccatgca tcgataagct tgatatcgaa ttccgcccct 2821 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt 2881 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac 2941 ctggcctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca 3001 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac 3061 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg 3121 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt 3181 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct 3241 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg 3301 ctttacgtgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg 3361 gttttccttt gaaaaacacg atgataatat ggccacaacc atgggttcta gattagataa 3421 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac 3481 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa 3541 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca 3601 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt 3661 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac 3721 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc 3781 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt 3841 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat 3901 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt 3961 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg 4021 gtccgcgtac agccgcgcgc gtacgaaaaa caattacggg tctaccatcg agggcctgct 4081 cgatctcccg gacgacgacg cccccgaaga ggcggggctg gcggctccgc gcctgtcctt 4141 tctccccgcg ggacacacgc gcagactgtc gacggccccc ccgaccgatg tcagcctggg 4201 ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga 4261 tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga 4321 ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga 4381 tgcccttgga attgacgagt acggtgggta gggggcgcga ggatcggaat tgaatatcga 4441 atgcagcagc aattcgtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc 4501 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat 4561 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg 4621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact 4681 ccctatcagt gatagagaaa agtgaaagtc agtttacact cctatcagtg atagagaaaa 4741 gtgaaagtcg agctcggtac ccgggtcgag taggcgtgta cggtgggagg cctatataag 4801 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccaacgc tgttttgcct 4861 ccaaaagaca ccgggaccga ggccgcctcg gccatgaaga tggacaaaaa gactatagtt 4921 tggtttagaa gagacctaag gattgaggat aatcctgcat tagcagcagc tgctcacgaa 4981 ggatctgttt ttcctgtctt catttggtgt cctgaagaag aaggacagtt ttatcctgga 5041 agagcttcaa gatggtggat gaaacaatca cttgctcact tatctcaatc cttgaaggct 5101 cttggatctg acctcacttt aatcaaaacc cacaacacga tttcagcgat cttggattgt 5161 atccgcgtta ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta 5221 gttcgggacc ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt gcaaagctac 5281 aatggagatc tattgtatga accgtgggag atatactgcg aaaagggcaa accttttacg 5341 agtttcaatt cttactggaa gaaatgctta gatatgtcga ttgaatccgt tatgcttcct 5401 cctccttggc ggttgatgcc aataactgca gcggctgaag cgatttgggc gtgttcgatt 5461 gaagaactag ggctggagaa tgaggccgag aaaccgagca atgcgttgtt aactagagct 5521 tggtctccag gatggagcaa tgctgataag ttactaaatg agttcatcga gaagcagttg 5581 atagattatg caaagaacag caagaaagtt gttgggaatt ctacttcact actttctccg 5641 tatctccatt tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa 5701 attatatggg caagagataa gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg 5761 ggaatcggtt taagagagta ttctcggtat atatgtttca acttcccgtt tactcacgag 5821 caatcgttgt tgagtcatct tcggtttttc ccttgggatg ctgatgttga taagttcaag 5881 gcctggagac aaggcaggac cggttatccg ttggtggatg ccggaatgag agagctttgg 5941 gctaccggat ggatgcataa cagaataaga gtgattgttt caagctttgc tgtgaagttt 6001 cttctccttc catggaaatg gggaatgaag tatttctggg atacactttt ggatgctgat 6061 ttggaatgtg acatccttgg ctggcagtat atctctggga gtatccccga tggccacgag 6121 cttgatcgct tggacaatcc cgcgttacaa ggcgccaaat atgacccaga aggtgagtac 6181 ataaggcaat ggcttcccga gcttgcgaga ttgccaactg aatggatcca tcatccatgg 6241 gacgctcctt taaccgtact caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa 6301 cccattgtag acatcgacac agctcgtgag ctactagcta aagctatttc aagaacccgt 6361 gaagcacaga tcatgatcgg agcagcagcc cgggatccac cggtcgccac catggtgagc 6421 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 6481 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 6541 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 6601 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 6661 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 6721 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 6781 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 6841 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 6901 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 6961 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 7021 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 7081 ggccgccact ccaccggcgg catggacgag ctgtacaagg gtggaggagg tagtggagga 7141 ggaggatcag ctgccatccg gaagaaactg gtgattgttg gtgatggagc ctgtggaaag 7201 acatgcttgc tcatagtctt cagcaaggac cagttcccag aggtgtatgt gcccacagtg 7261 tttgagaact atgtggcaga tatcgaggtg gatggaaagc aggtagagtt ggctttgtgg 7321 gacacagctg ggcaggaaga ttatgaccgc ctgaggcccc tctcctaccc agataccgat 7381 gttatactga tgtgtttttc catcgacagc cctgatagtt tagaaaacat cccagaaaag 7441 tggaccccag aagtcaagca tttctgtccc aacgtgccca tcatcctggt tgggaataag 7501 aaggatcttc ggaatgatga gcacacaagg cgggagctag ccaagatgaa gcaggagccg 7561 gtgaaacctg aagaaggcag agatatggca aacaggattg gcgcttttgg gtacatggag 7621 tgttcagcaa agaccaaaga tggagtgaga gaggtttttg aaatggctac gagagctgct 7681 ctgcaagcta gacgtgggaa gaaaaaatct gggtgccttg tcttgtgaaa ccttgctgga 7741 agcacagccc ttatgcggta agtttaaacg gatccactag ttctagaggc cgcggccgcg 7801 gatccggatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga 7861 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt 7921 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag 7981 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca 8041 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa 8101 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca 8161 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga 8221 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg 8281 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt 8341 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca 8401 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg 8461 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg 8521 tcagcggggg tctttcacac atgcagcatg tatcaaaatt aatttggttt tttttcttaa 8581 gtatttacat taaatggcca tagtacttaa agttacattg gcttccttga aataaacatg 8641 gagtattcag aatgtgtcat aaatctgcat taatgaatcg gccaacgcgc ggggagaggc 8701 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 8761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 8821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 8881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 8941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 9001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 9061 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 9121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 9181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 9241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 9301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 9361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 9421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 9481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 9541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 9601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 9661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 9721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 9781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 9841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 9901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 9961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 10021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 10081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 10141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 10201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 10261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 10321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 10381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 10441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 10501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 10561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 10621 ccgcgcacat ttccccgaaa agtgccacct gacgtc //