LOCUS Exported 10249 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEV53D SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10249) AUTHORS Patel M, Giddings AM, Sechelski J, Olsen JC TITLE High efficiency gene transfer to airways of mice using influenza hemagglutinin pseudotyped lentiviral vectors. JOURNAL J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695. PUBMED 23319179 REFERENCE 2 (bases 1 to 10249) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10249 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7464..7481) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7470..7694 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(7827..7846) /label=F1ori-R /note="F1 origin, reverse primer" polyA_signal 7922..8043 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7959..7978) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8013..8032 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8092..8108) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8092..8108) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8105..8127) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8116..8132 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8140..8170) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8185..8206 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8323..8340) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8494..9082) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8574..8593) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9253..10113) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9876..9895 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10114..10218) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 cgcgggcgcg ccaggtaaga tgggagaccc tttgacatgg agcaaggcgc tcaagaagtt 961 agagaaggtg acggtacaag ggtctcagaa attaactact ggtaactgta attgggcgct 1021 aagtctagta gacttatttc atgataccaa ctttgtaaaa gaaaaggact ggcagctgag 1081 ggatgtcatt ccattgctgg aagatgtaac tcagacgctg tcaggacaag aaagagaggc 1141 ctttgaaaga acatggtggg caatttctgc tgtaaagatg ggcctccaga ttaataatgt 1201 agtagatgga aaggcatcat tccagctcct aagagcgaaa tatgaaaaga agactgctaa 1261 taaaaagcag tctgagccct ctgaagaata tccaatcatg atagatggga ctggaaacag 1321 aaattttaga cctctaacac ctagaggata tactacttgg gtgaatacca tacagacaaa 1381 tggtctatta aatgaagcta gtcaaaactt atttgggata ttatcagtag actgtacttc 1441 tgaagaaatg aatgcatttt tggatgtggt acctggccag gcaggacaaa agcagatatt 1501 acttgatgca attgataaaa tagcagatga ttgggataat agacatccat taccgaatgc 1561 tccactggtg gcaccaccac aagggcctat tcccatgaca gcaaggttta ttagaggttt 1621 aggagtacct agagaaagac agatggagcc tgcttttgat cagtttaggc agacatatag 1681 acaatggata atagaagcca tgtcagaagg catcaaagtg atgattggaa aacctaaagc 1741 tcaaaatatt aggcaaggag ctaaggaacc ttacccagaa tttgtagaca gactattatc 1801 ccaaataaaa agtgagggac atccacaaga gatttcaaaa ttcttgactg atacactgac 1861 tattcagaac gcaaatgagg aatgtagaaa tgctatgaga catttaagac cagaggatac 1921 attagaagag aaaatgtatg cttgcagaga cattggaact acaaaacaaa agatgatgtt 1981 attggcaaaa gcacttcaga ctggtcttgc gggcccattt aaaggtggag ccttaaaagg 2041 agggccacta aaggcagcac aaacatgtta taactgtggg aagccaggac atttatctag 2101 tcaatgtaga gcacctaaag tctgttttaa atgtaaacag cctggacatt tctcaaagca 2161 atgcagaagt gttccaaaaa acgggaagca aggggctcaa gggaggcccc agaaacaaac 2221 tttcccgata caacagaaga gtcagcacaa caaatctgtt gtacaagaga ctcctcagac 2281 tcaaaatctg tacccagatc tgagcgaaat aaaaaaggaa tacaatgtca aggagaagga 2341 tcaagtagag gatctcaacc tggacagttt gtgggagtaa catataatct agagaaaagg 2401 cctactacaa tagtattaat taatgatact cccttaaatg tactgttaga cacaggagca 2461 gatacttcag tgttgactac tgcacattat aataggttaa aatatagagg gagaaaatat 2521 caagggacgg gaataatagg agtgggagga aatgtggaaa cattttctac gcctgtgact 2581 ataaaggaaa agggtagaca cattaagaca agaatgctag tggcagatat tccagtgact 2641 attttgggac gagatattct tcaggactta ggtgcaaaat tggttttggc acagctctcc 2701 aaggaaataa aatttagaaa aatagagtta aaagagggca caatggggcc aaaaattcct 2761 caatggccac tcactaagga gaaactagaa ggggctaaag agatagtcca aagactattg 2821 tcagagggaa aaatatcaga agctagtgac aataatcctt ataattcacc catatttgta 2881 ataaaaaaga ggtctggcaa atggaggtta ttacaagatc tgagagaatt aaacaaaaca 2941 gtacaagtag gaacggaaat atccagagga ttgcctcacc cgggaggatt aattaaatgt 3001 aaacacatga ctgtattaga tattggagat gcatatttca ctataccctt agatccagag 3061 tttagaccat atacagcttt cactattccc tccattaatc atcaagaacc agataaaaga 3121 tatgtgtgga attgtttacc acaaggattc gtgttgagcc catatatata tcagaaaaca 3181 ttacaggaaa ttttacaacc ttttagggaa agatatcctg aagtacaatt gtatcaatat 3241 atggatgatt tgttcgtggg aagtaatggt tctaaaaaac aacacaaaga gttaatcata 3301 gaattaaggg caatcttact ggaaaagggt tttgagacac cagatgataa attacaagaa 3361 gtgccacctt atagctggct aggttatcaa ctttgtcctg aaaattggaa agtacaaaaa 3421 atgcaattag acatggtaaa gaatccaacc cttaatgatg tgcaaaaatt aatggggaat 3481 ataacatgga tgagctcagg ggtcccaggg ttgacagtaa aacacatagc agctactact 3541 aagggatgtt tagagttgaa tcaaaaagta atttggacgg aagaggcaca aaaagagtta 3601 gaagaaaata atgagaagat taaaaatgct caagggttac aatattataa tccagaagaa 3661 gaaatgttat gtgaggttga aattacaaaa aattatgagg caacttatgt tataaaacaa 3721 tcacaaggaa tcctatgggc aggtaaaaag attatgaagg ctaataaggg atggtcaaca 3781 gtaaaaaatt taatgttact gttgcaacat gtggcaacag aaagtattac tagagtagga 3841 aaatgtccaa cgtttaaggt accatttacc aaagagcaag taatgtggga aatgcaaaaa 3901 ggatggtatt attcttggct cccagaaata gtatatacac atcaagtagt tcatgatgat 3961 tggagaatga aattggtaga agaacctaca tcaggaataa caatatacac tgatggggga 4021 aaacaaaatg gagaaggaat agcagcttat gtgaccagta atgggagaac taaacagaaa 4081 aggttaggac ctgtcactca tcaagttgct gaaagaatgg caatacaaat ggcattagag 4141 gataccagag ataaacaagt aaatatagta actgatagtt attattgttg gaaaaatatt 4201 acagaaggat taggtttaga aggaccacaa agtccttggt ggcctataat acaaaatata 4261 cgagaaaaag agatagttta ttttgcttgg gtacctggtc acaaagggat atgtggtaat 4321 caattggcag atgaagccgc aaaaataaaa gaagaaatca tgctagcata ccaaggcaca 4381 caaattaaag agaaaagaga tgaagatgca gggtttgact tatgtgttcc ttatgacatc 4441 atgatacctg tatctgacac aaaaatcata cccacagatg taaaaattca agttcctcct 4501 aatagctttg gatgggtcac tgggaaatca tcaatggcaa aacaggggtt attaattaat 4561 ggaggaataa ttgatgaagg atatacagga gaaatacaag tgatatgtac taatattgga 4621 aaaagtaata ttaaattaat agagggacaa aaatttgcac aattaattat actacagcat 4681 cactcaaatt ccagacagcc ttgggatgaa aataaaatat ctcagagagg ggataaagga 4741 tttggaagta caggagtatt ctgggtagaa aatattcagg aagcacaaga tgaacatgag 4801 aattggcata catcaccaaa gatattggca agaaattata agataccatt gactgtagca 4861 aaacagataa ctcaagaatg tcctcattgc actaagcaag gatcaggacc tgcaggttgt 4921 gtcatgagat ctcctaatca ttggcaggca gattgcacac atttggacaa taagataata 4981 ttgacttttg tagagtcaaa ttcaggatac atacatgcta cattattgtc aaaagaaaat 5041 gcattatgta cttcattggc tattttagaa tgggcaagat tgttttcacc aaagtcctta 5101 cacacagata acggcactaa ttttgtggca gaaccagttg taaatttgtt gaagttccta 5161 aagatagcac ataccacagg aataccatat catccagaaa gtcagggtat tgtagaaagg 5221 gcaaatagga ccttgaaaga gaagattcaa agtcatagag acaacactca aacactggag 5281 gcagctttac aacttgctct cattacttgt aacaaaggga gggaaagtat gggaggacag 5341 acaccatggg aagtatttat cactaatcaa gcacaagtaa tacatgagaa acttttacta 5401 cagcaagcac aatcctccaa aaaattttgt ttttacaaaa tccctggtga acatgattgg 5461 aagggaccta ctagggtgct gtggaagggt gatggtgcag tagtagttaa tgatgaagga 5521 aagggaataa ttgctgtacc attaaccagg actaagttac taataaagcc aaattgagta 5581 ttgttgcagg aagcaagacc caactaccat tgtcagctgt gtttcctgag gtctctagga 5641 attgattacc tcgatgcttc attaaggaag aagaataaac aaagactgaa ggcaatccaa 5701 caaggaagac aacctcaata tttgttataa ggtttgatat atgggattat ttggtaaagg 5761 ggtaacatgg tcagcatcgc attctatggg gggatcccag ggggaatctc aacccctatt 5821 acccaacagt cagaaaaatc taagtgtgag gagaacacaa tgtttcaacc ttattgttat 5881 aataatgaca gtaagaacag catggcagaa tcgaaggaag caagagacca agaaatgaac 5941 ctgaaagaag aatctaaaga agaaaaaaga agaaatgact ggtggaaaaa aggtatgttt 6001 ctgttatgct tagcaggaac tactggagga atactttggt ggtatgaagg actcccacag 6061 caacattata tagggttggt ggcgataggg ggaagattaa acggatctgg ccaatcaaat 6121 gctatagaat gctggggttc cttcccgggg tgtagaccat ttcaaaatta cttcagttat 6181 gagaccaata gaagcatgca tatggataat aatactgcta cattattaga agctttaacc 6241 aatataactg ctctataaat aacaaaacag aattagaaac atggaagtta gtaaagactt 6301 ctggcgtaac tcctttacct atttcttctg aagctaacac tggactaatt agacataaga 6361 gagattttgg tataagtgca atagtggcag ctattgtagc cgctactgct attgctgcta 6421 gcgctactat gtcttatgtt gctctaactg aggttaacaa aataatggaa gtacaaaatc 6481 atacttttga ggtagaaaat agtactctaa atggtatgga tttaatagaa cgacaaataa 6541 agatattata tgctatgatt cttcaaacac atgcagatgt tcaactgtta aaggaaagac 6601 aacaggtaga ggagacattt aatttaattg gatgtataga aagaacacat gtattttgtc 6661 atactggtca tccctggaat atgtcatggg gacatttaaa tgagtcaaca caatgggatg 6721 actgggtaag caaaatggaa gatttaaatc aagagatact aactacactt catggagcta 6781 ggaacaattt ggcacaatcc atgataacat tcaatacacc agatagtata gctcaatttg 6841 gaaaagacct ttggagtcat attggaaatt ggattcctgg attgggagct tccattataa 6901 aatatatagt gatgtttttg cttatttatt tgttactaac ctcttcgcct aagatcctca 6961 gggccctctg gaaagtgacc agtggtgcag ggtcctccgg cagtcgttac ctgaagaaaa 7021 aattccatca caaacatgca tcgcgagaag acacctggga ccaggcccaa cacaacatac 7081 acctagcagg cgtgaccggt ggatcagggg acaaatacta caaacagaag tactccagga 7141 acgactggaa tggagaatca gaggagtaca acaggcggcc aaagagctgg gtgaagtcaa 7201 tcgaggcatt tggagagagc tatatttccg agaagaccaa aggggagatt tctcagcctg 7261 gggcggctat caacgagcac aagaacggct ctggggggaa caatcctcac caagggtcct 7321 tagacctgga gattcgaagc gaaggaggaa acatttatga ctgttgcatt aaagcccaag 7381 aaggaactct cgctatccct tgctgtggat ttcccttatg gctattttgg ggactagagg 7441 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 7501 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 7561 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 7621 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 7681 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 7741 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 7801 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 7861 gttcgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 7921 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 7981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 8041 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 8101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 8161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 8221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 8281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 8341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 8401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 8461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 8521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 8581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 8641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg 8701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 8761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 8821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 8881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 8941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 9001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 9061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 9121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 9181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 9241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 9301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 9361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 9421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 9481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 9541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 9601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 9721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 9781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 9841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 9901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 9961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 10021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 10081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 10141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 10201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc //