LOCUS Exported 5486 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pPAiRFP1-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5486) AUTHORS Piatkevich KD, Subach FV, Verkhusha VV TITLE Far-red light photoactivatable near-infrared fluorescent proteins engineered from a bacterial phytochrome. JOURNAL Nat Commun. 2013 Jul 10;4:2153. doi: 10.1038/ncomms3153. PUBMED 23842578 REFERENCE 2 (bases 1 to 5486) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5486 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 616..625 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 2274..2395 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2311..2330) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2365..2384 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2402..2857) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2539..2560) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2751..2770 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2884..2988 /gene="bla" /label=AmpR promoter promoter 2990..3347 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3013..3033) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3198..3333 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3260..3279 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3382..4176 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3436..3455) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4046..4065 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4364..4383) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4408..4455 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4784..5372 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5273..5292 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgccac catggcatcc acggactatc atgtcgacct gaccaattgc 661 gaccgcgaac ccattcatat tccaggatat attcaaccgc atggctgcct catcgcctgc 721 gataacgcca tgcgaatggt gctgcggcat tccgaaaact gcggcgaact gctgggactt 781 gagggcgatc tcaacggcag gaccgccgag gatgtgctcg gcaaaaagct cgtccacgat 841 ctccgcaacg cgctcaccgt caccggtaga accacgcgcc ccgccatgct gcctgcaatg 901 gaaacaagcg acggccgcag cttcgatatt tcactccatc gctacaaatc caccaccatc 961 atcgaattcg agccctcggg cagcgacgcg cagccgctcg acacggcgcg caagatggtg 1021 gaccgcatcc gcgaagccga cagggtcgaa agcctgatct ccagaaccac acgcctggta 1081 aaggcgacgc tgggttacga ccgggtgctg atctaccgct tcctggaaga cggcgccggc 1141 aaggtcgtgt cggaggccaa gcagcccgag ctggagagct ttctcgggca atatttcccc 1201 gccagcgata ttccacagca ggctcgcgta ctttatctca agaacacctt gcgtatcatc 1261 tccgatgcca gcagcacccc cattccggtc ctaccagccg tcgatgtctc cggtgagccg 1321 ctcgatctct cttacgcgca tttgcgcagc ttctcgccca tccattgcga atatctccgc 1381 aatatgggtg ttgccgcctc catgtcgatt tctgtgattg tcgatggcgc cctgtggggc 1441 ctgatcgtct gccaccattg ttcgccgcgt gtgctgagca tgccagttcg gatcgctgcg 1501 gtcatgttcg gggaattttt ctccatgcgt ctgcaggttc tgaagcagaa gcgccgcctc 1561 gacaccatca accatgccca tgcggcgctc gaccggtttc tgcggctggc cgcccatcac 1621 gccaacattg aagaactgct ggttgacagt tttcaggatt ttgccgatct gatgccctgc 1681 gacggtgtcg ggctgtgggt cggcaacaac tggcacggcc atggcgcaac gccaccgcat 1741 gatgccattc caaggctggc gcgtttcgtc gcttcggttt cggaaggcag ggtctgggca 1801 acacacgccc tgtcgcaggc cataccggaa gcggaaatat acgccggcac cgcggccgga 1861 atgcttgcta tcccgctttc gcaggtcaag agcgactatc tcctgttttt ccgcaaggag 1921 atcgtgcaga acctgaactg ggccggcaat ccggaaaaat cctatgaaac cggcccgatg 1981 ggcgaccgcc tcacgccgcg taaaagcttc gcaatctgga aagagaccgt tcgcctgcag 2041 gcacagccct ggtcggaagt cgaccgggaa atcgccgaag ccgcgagaat cgcgcttgtc 2101 gaagtggcgt tctaccacag cgagttgatg gcaggcctag agtacaagta aagcggccgc 2161 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 2221 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 2281 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 2341 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 2401 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 2461 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 2521 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 2581 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 2641 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 2701 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 2761 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 2821 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 2881 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 2941 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 3001 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 3061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 3121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 3181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 3241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 3301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 3361 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 3421 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3481 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3541 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 3601 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3661 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 3721 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 3781 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 3841 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 3901 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 3961 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 4021 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 4081 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 4141 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 4201 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 4261 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 4321 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 4381 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 4441 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 4501 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 4561 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 4621 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 4681 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 4741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 4801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 4861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 4921 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5101 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 5161 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 5221 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 5281 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 5341 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 5401 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 5461 tggataaccg tattaccgcc atgcat //