LOCUS Exported 4026 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUC-UAS1B11-Leum SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4026) AUTHORS Blazeck J, Liu L, Redden H, Alper H TITLE Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. JOURNAL Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16. PUBMED 21926196 REFERENCE 2 (bases 1 to 4026) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4026 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 364..386 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 378..395 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1805..1821) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1805..1821) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1818..1840) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1829..1845 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1853..1883) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1898..1919 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2036..2053) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2207..2795) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2287..2306) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2966..3826) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3589..3608 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3827..3931) /gene="bla" /label=AmpR promoter primer_bind 3999..4017 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtttcga actgcagttg aattcctgag 421 gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt gtagtgtacg 481 tacattatcg agaccgttgt tcccgcccac ctcgatccgg tctagactga ggtgtctcac 541 aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac gtacattatc 601 gagaccgttg ttcccgccca cctcgatccg gggatccctg aggtgtctca caagtgccgt 661 gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta cgtacattat cgagaccgtt 721 gttcccgccc acctcgatcc gggtcgacct gaggtgtctc acaagtgccg tgcagtcccg 781 cccccacttg cttctctttg tgtgtagtgt acgtacatta tcgagaccgt tgttcccgcc 841 cacctcgatc cgggagctcc tgaggtgtct cacaagtgcc gtgcagtccc gcccccactt 901 gcttctcttt gtgtgtagtg tacgtacatt atcgagaccg ttgttcccgc ccacctcgat 961 ccggtctaga ctgaggtgtc tcacaagtgc cgtgcagtcc cgcccccact tgcttctctt 1021 tgtgtgtagt gtacgtacat tatcgagacc gttgttcccg cccacctcga tccggggatc 1081 cctgaggtgt ctcacaagtg ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag 1141 tgtacgtaca ttatcgagac cgttgttccc gcccacctcg atccgggtcg acctgaggtg 1201 tctcacaagt gccgtgcagt cccgccccca cttgcttctc tttgtgtgta gtgtacgtac 1261 attatcgaga ccgttgttcc cgcccacctc gatccgggca tgcggtaccc gaattcgagc 1321 tcctactcta gactgaggtg tctcacaagt gccgtgcagt cccgccccca cttgcttctc 1381 tttgtgtgta gtgtacgtac attatcgaga ccgttgttcc cgcccacctc gatccgggga 1441 tccctgaggt gtctcacaag tgccgtgcag tcccgccccc acttgcttct ctttgtgtgt 1501 agtgtacgta cattatcgag accgttgttc ccgcccacct cgatccgggt cgacctgagg 1561 tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc tctttgtgtg tagtgtacgt 1621 acattatcga gaccgttgtt cccgcccacc tcgatccggg catgcactga tcacgggcaa 1681 aagtgcgtat atatacaaga gcgtttgcca gccacagatt ttcactccac acaccacatc 1741 acacatacaa ccacacacat ccacaatgga acccgaaact aaaagcttca ggcgcgccat 1801 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 1861 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 1921 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 1981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 2041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 2101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 2161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 2221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 2281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 2521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 2581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 2641 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 2701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 2761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 2821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 2881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 2941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 3001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 3061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 3121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 3181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 3241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 3301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 3541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 3601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 3661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 3721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 3781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 3841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 3901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 3961 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 4021 ttcgtc //