LOCUS Exported 462 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Non-integrating (episomal) expression of EGFP. ACCESSION . VERSION . KEYWORDS pCE-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 462) AUTHORS Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S TITLE An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. JOURNAL Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293. PUBMED 23193063 REFERENCE 2 (bases 1 to 462) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..462 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 15..24 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(52..71) /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind complement(133..155) /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" ORIGIN 1 tctgcccttg ctcaccatgg tggcgaattc tttgccaaaa tgatgagaca gcacaataac 61 cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc 121 tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag 181 gcagccgtcc ccccgcggac agccccgagg ctggagaggg agaaggggac ggcggcgcgg 241 cgacgcacga aggccctccc cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc 301 gcgcccgcta gagggggtgc ggcggcgcct cccagatttc ggctccgcac agatttggga 361 caaaggaagt ccctgcgccc tctcgcacga ttaccataaa aggcaatggc tgcggctcgc 421 cgcgcctcga cagccgccgg cgctccgggg gccgccgcgc cc //