LOCUS       Exported                 892 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Encodes a gRNA that targets Can1.Y in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    p426-SNR52p-gRNA.CAN1.Y-SUP4t
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     11..31
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13..31
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        49..317
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        338..412
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      417..436
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      442..689
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(707..726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(708..726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(736..753)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(736..752)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(745..767)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagcttc tttgaaaaga
       61 taatgtatga ttatgctttc actcatattt atacagaaac ttgatgtttt ctttcgagta
      121 tatacaaggt gattacatgt acgtttgaag tacaactcta gattttgtag tgccctcttg
      181 ggctagcggt aaaggtgcgc attttttcac accctacaat gttctgttca aaagattttg
      241 gtcaaacgct gtagaagtga aagttggtgc gcatgtttcg gcgttcgaaa cttctccgca
      301 gtgaaagata aatgatcgat acgttctcta tggaggagtt ttagagctag aaatagcaag
      361 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tggtgctttt
      421 tttgtttttt atgtcttcga gtcatgtaat tagttatgtc acgcttacgt tcacgccctc
      481 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
      541 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
      601 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
      661 ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc aattcgccct atagtgagtc
      721 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      781 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      841 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gg
//
