LOCUS Exported 4701 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBAD/HisD-TagRFP675 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4701) AUTHORS Piatkevich KD, Malashkevich VN, Morozova KS, Nemkovich NA, Almo SC, Verkhusha VV TITLE Extended Stokes shift in fluorescent proteins: chromophore-protein interactions in a near-infrared TagRFP675 variant. JOURNAL Sci Rep. 2013;3:1847. doi: 10.1038/srep01847. PUBMED 23677204 REFERENCE 2 (bases 1 to 4701) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4701 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..285 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 208..227 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" CDS 328..345 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(1102..1119) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 1272..1358 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1450..1477 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 1496..1587 /gene="bla" /label=AmpR promoter CDS 1588..2448 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1806..1825) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2619..3207 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3108..3127 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3361..3378 /label=L4440 /note="L4440 vector, forward primer" misc_feature 3393..3533 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(3459..3478) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3619..3641 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(3797..4675) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" ORIGIN 1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc 301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcag atctatgagc 361 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac 421 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gacccaaaga 481 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc 541 atgtacggca gcaagacctt catcaaccac acccagggca tccccgactt ctggaagcag 601 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg 661 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga 721 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc 781 aacaccgaga tgatgtaccc cgctgacggc ggcctggaag gcagaaacta catggccctg 841 aagctcgtgg gcgggggcca cctgatctgc agcttgaaga ccacatacag atccaagaaa 901 cccgctaaga acctcaagat gcccggcgtc tactatgtgg accgcagact ggaaagaatc 961 aaggaggccg acaaagagac ctatgtcgag cagcacgagg tggctgtggc cagatactgc 1021 gacctcccta gcaaactggg gcacaagctt aattaagaat tcgaagcttg gctgttttgg 1081 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat 1141 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc 1201 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa 1261 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 1321 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg 1381 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc 1441 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt 1501 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 1561 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 1621 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 1681 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 1741 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 1801 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc 1861 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 1921 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 1981 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 2041 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 2101 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 2161 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 2221 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 2281 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 2341 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 2401 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 2461 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 2521 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 2581 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 2641 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 2701 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 2761 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 2821 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 2881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 2941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 3001 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 3061 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 3121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 3181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 3241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 3301 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 3361 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat 3421 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca 3481 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac 3541 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 3601 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 3661 aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca taatgtgcct 3721 gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg 3781 tctgattcgt taccaattat gacaacttga cggctacatc attcactttt tcttcacaac 3841 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga 3901 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca 3961 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta 4021 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc 4081 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta 4141 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca 4201 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt 4261 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga 4321 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac 4381 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa 4441 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat 4501 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg 4561 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca 4621 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt 4681 tcatactccc gccattcaga g //