LOCUS Exported 919 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pCDNA3-LOV-VP16 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 919) AUTHORS Yazawa M, Sadaghiani AM, Hsueh B, Dolmetsch RE TITLE Induction of protein-protein interactions in live cells using light. JOURNAL Nat Biotechnol. 2009 Oct;27(10):941-5. doi: 10.1038/nbt.1569. Epub 2009 Oct 4. PUBMED 19801976 REFERENCE 2 (bases 1 to 919) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..919 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 54..73 /label=T7 /note="T7 promoter, forward primer" promoter 54..72 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 104..113 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 116..142 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 668..778 /codon_start=1 /product="3 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)" /label=VP48 /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML" promoter complement(822..840) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(823..840) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(860..877) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" ORIGIN 1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg 61 actcactata gggagaccca agcttggtac cgagctcgga tccgccacca tggcttaccc 121 atacgatgtt ccagattacg ctgcgagaga acatgcgatc ggagaagcca ccggaaaacg 181 aaagaaacgc ggcagagttg aagaagcaga agaatactgt aacgatggaa tcgaagaaca 241 agtagaggat gagaagcttc cgttagaggt tgggatgttc tattacccaa tgactccgcc 301 ttcgttcatt gtttccgatg ctctggagcc agattttcct ttgatctatg tcaacagagt 361 cttcgaagtc ttcactggct atcgtgccga tgaagttctt ggtcgtaact gtcgattcct 421 acagtacaga gatcctcgag ctcaaaggcg tcacccattg gttgatcctg tggttgtatc 481 tgagattagg agatgtcttg aagaaggtat tgaattccaa gacgagcttc ttaatttcag 541 aaaagatggt actcctttgg ttaacagact acggcttgct ccaatacgtg acgatgatgg 601 aaccattaca cacgtaattg ggatacaggt cttctctgaa acgggtaccg aaagcggcgg 661 gccggccgac gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga 721 ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc 781 cgggtaagcg gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat 841 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 901 ccctcccccg tgccttcct //