LOCUS Exported 4822 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS XNOR-gate-V2.0-ASV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4822) AUTHORS Bonnet J, Yin P, Ortiz ME, Subsoontorn P, Endy D TITLE Amplifying genetic logic gates. JOURNAL Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28. PUBMED 23539178 REFERENCE 2 (bases 1 to 4822) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4822 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..22 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" terminator 185..212 /label=T7Te terminator /note="phage T7 early transcription terminator" terminator 228..299 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_RNA 413..462 /label=sTRSV HHRz /note="hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)" RBS 556..564 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 571..1284 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGTKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" misc_feature 1327..1347 /label=BioBrick suffix /note="universal suffix for all parts" terminator 1383..1414 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" terminator 1539..1633 /label=lambda t0 terminator /note="transcription terminator from phage lambda" rep_origin 2125..2347 /label=pSC101 ori /note="low-copy replication origin that requires the Rep101 protein" CDS 2395..3345 /codon_start=1 /gene="rep101" /product="RepA protein needed for replication with the pSC101 origin" /label=Rep101 /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI" CDS 3634..4494 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" terminator 4624..4653 /label=T3Te terminator /note="phage T3 early transcription terminator" terminator 4747..4776 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 gaattcgcgg ccgcttctag agaaaaaatt tatttgcttt cgcatctttt tgtacctata 61 atgtgtggaa agcttatgcc aacacaatta acatctcaat caaggtaaat gctttttgct 121 ttttttgctc ggccggcttg tcgacgacgg cggtctccgt cgtcaggatc atccgggcta 181 taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc 241 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga 301 tgcctggggg tttgtaccgt acaccactga gaccgcggtg gttgaccaga caaaccacga 361 aaaggagttt tttagttacc ttaattgaaa taaacgaaat aaaaactcgc agctgtcacc 421 ggatgtgctt tccggcctga tgagtccgtg aggacgaaac agcctctaca aataattttg 481 tttaagggcc caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa 541 acatcttaat catgctaagg aggttttcta atgcgtaaag gcgaagagct gttcactggt 601 gtcgtcccta ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt 661 ggcgagggtg aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact 721 ggtaaactgc cggtaccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc 781 tttgctcgtt atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa 841 ggctatgtgc aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg 901 gaagtgaaat ttgaaggcga tactctggta aaccgcattg agctgaaagg cattgacttt 961 aaagaagacg gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt 1021 tacatcaccg ccgataaaca aaaaaatggc actaaagcga attttaaaat tcgccacaac 1081 gtggaggatg gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat 1141 ggtcctgttc tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat 1201 ccgaacgaga aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg 1261 catggtatgg atgaactgta caaagccggc gctgctaacg acgaaaacta cgctgcgtct 1321 gtttaatact agtagcggcc gctgcaggag tcactaaggg ttagttagtt agattagcag 1381 aaagtcaaaa gcctccgacc ggaggctttt gactaaaact tcccttgggg ttatcattgg 1441 ggctcactca aaggcggtaa tcagataaaa aaaatcctta gctttcgcta aggatgattt 1501 ctgctagagc tgtcagacca agtttacgag ctcgcttgga ctcctgttga tagatccagt 1561 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt 1621 tattggtgag aatccaagca ctagggacag taagacgggt aagcctgttg atgataccgc 1681 tgccttactg ggtgcattag ccagtctgaa tgacctgtca cgggataatc cgaagtggtc 1741 agactggaaa atcagagggc aggaactgct gaacagcaaa aagtcagata gcaccacata 1801 gcagacccgc cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta 1861 gtttccttgc atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca 1921 gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg 1981 tcggagacaa aaggaatatt cagcgatttg cccgagcttg cgagggtgct acttaagcct 2041 ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa 2101 tactgcggaa ctgactaaag tagtgagtta tacacagggc tgggatctat tctttttatc 2161 tttttttatt ctttctttat tctataaatt ataaccactt gaatataaac aaaaaaaaca 2221 cacaaaggtc tagcggaatt tacagagggt ctagcagaat ttacaagttt tccagcaaag 2281 gtctagcaga atttacagat acccacaact caaaggaaaa ggacatgtaa ttatcattga 2341 ctagcccatc tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct 2401 gaattagttg ttttcaaagc aaatgaacta gcgattagtc gctatgactt aacggagcat 2461 gaaaccaagc taattttatg ctgtgtggca ctactcaacc ccacgattga aaaccctaca 2521 aggaaagaac ggacggtatc gttcacttat aaccaatacg ctcagatgat gaacatcagt 2581 agggaaaatg cttatggtgt attagctaaa gcaaccagag agctgatgac gagaactgtg 2641 gaaatcagga atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag 2701 ttctcaagcg aaaaattaga attagttttt agtgaagaga tattgcctta tcttttccag 2761 ttaaaaaaat tcataaaata taatctggaa catgttaagt cttttgaaaa caaatactct 2821 atgaggattt atgagtggtt attaaaagaa ctaacacaaa agaaaactca caaggcaaat 2881 atagagatta gccttgatga atttaagttc atgttaatgc ttgaaaataa ctaccatgag 2941 tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc 3001 aatatgaaat tggtggttga taagcgaggc cgcccgactg atacgttgat tttccaagtt 3061 gaactagata gacaaatgga tctcgtaacc gaacttgaga acaaccagat aaaaatgaat 3121 ggtgacaaaa taccaacaac cattacatca gattcctacc tacgtaacgg actaagaaaa 3181 acactacacg atgctttaac tgcaaaaatt cagctcacca gttttgaggc aaaatttttg 3241 agtgacatgc aaagtaagca tgatctcaat ggttcgttct catggctcac gcaaaaacaa 3301 cgaaccacac tagagaacat actggctaaa tacggaagga tctgaggttc ttatggctct 3361 tgtatctatc agtgaagcat caagactaac aaacaaaagt agaacaactg ttcaccgtta 3421 gatatcaaag ggaaaactgt ccatatgcac agatgaaaac ggtgtaaaaa agatagatac 3481 atcagagctt ttacgagttt ttggtgcatt taaagctgtt caccatgaac agatcgacaa 3541 tgtaactact agagctaaat acattcaaat atctatccgc tcatgagaca ataaccctga 3601 taaatgcttc aataatattg aaaaaggaag aatatgagta ttcaacattt ccgtgtcgcc 3661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 3721 aaagtaaaag atgccgaaga tcagttgggt gcacgtgtgg gttacatcga actggacctc 3781 aacagcggta agattcttga gagttttcgc cccgaagaac gtttcccaat gatgagcact 3841 tttaaagttc tgctctgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 3901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 3961 catcttacgg acggcatgac agtacgcgaa ttatgcagcg ctgccataac catgagtgat 4021 aacacggcgg ccaacttact tctgacaacg atcggaggac cgaaggagct taccgctttt 4081 ttgcacaaca tgggtgatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 4141 gccataccaa acgacgagcg tgacaccacg atgcctgtag ctatggcaac aacgttgcgc 4201 aaactcttaa ctggcgaact tcttactctc gcttcccggc aacaattaat agactggatg 4261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 4321 gctgataaat ctggagccgg tgagcgtggg tcccgcggta ttattgcagc cctggggcca 4381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagccaggc aactatggac 4441 gaacgtaatc gccagatcgc tgagataggt gcctccctga ttaagcattg gtaataatac 4501 tagctccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa 4561 aagattactt cgcgtttgcc acctgacgtc taagaaaagg aatattcagc aatttgcccg 4621 tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac gtaattagtt 4681 agttagccct tagtgactct gccacctgac gtctaagaaa aggaatattc agcaatttgc 4741 ccgtgccgaa gaaaggccca cccgtgaagg tgagccagtg agttgattgc tacgtaatta 4801 gttagttagc ccttagtgac tc //