LOCUS Exported 3173 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCFJ1272 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3173) TITLE Erik Jorgensen's plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 3173) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3173 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 283..299 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 283..299 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" protein_bind 319..339 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" misc_feature 339..446 /label=MCS /note="pBluescript multiple cloning site" primer_bind 363..379 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 363..379 /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(413..429) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(414..430) /label=pBluescriptKS /note="For pBluescript vector" protein_bind complement(446..466) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(474..491) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(474..490) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS complement(496..507) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(1058..1077) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1177..1199 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(1237..1255) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1323..1427 /gene="bla" /label=AmpR promoter CDS 1428..2288 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1646..1665) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2459..3047 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2948..2967 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat 61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt 121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac 181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt 241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc 301 atgattacgc caagctatca actttgtata gaaaagttga gctccaccgc ggtggcggcc 361 gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg 421 tcgacctcga gggggggccc ggtaccaact ttattataca tagttgataa ttcactggcc 481 gtcgttttac atggcacgcc cttctattac agggtttctg ataacaaagg gtgccggatc 541 tgcggcttac tcaccagacc tggccccatc cgattaccac ctattcgctt cgatgggaca 601 cgcactcgct gagcagcgct tcgattctta cgaaagtgtg aaaaaatggc tcgatgaatg 661 gttcgccgca aaagacgatg agttctactg gcgtggaatc cacaaattgc ccgagagatg 721 ggaaaaatgt gtagctagcg acggcaaata ctttgaataa atgatttttt ctttttccac 781 aaaatttaac gtgttttttg atttaaaaaa aacgacattt catacttgta cacctgatat 841 ctagatacct aggtgagctc tggtaccccc ctggcgttac ccaacttaat cgccttgcag 901 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 961 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc 1021 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 1081 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 1141 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 1201 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 1261 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 1321 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 1381 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 1441 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 1501 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 1561 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 1621 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 1681 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 1741 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 1801 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 1861 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 1921 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1981 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 2041 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 2101 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 2161 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 2221 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 2281 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 2341 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 2401 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 2461 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 2521 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 2581 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 2641 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2701 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2761 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2821 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2881 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2941 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 3001 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 3061 cggccttttt acggttcctg gtcttttgct ggccttttgc tcacatgttc tttcctgcgt 3121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat acc //