LOCUS       Exported               16397 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pIC26
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16397)
  AUTHORS   Cheeseman IM, Desai A
  TITLE     A combined approach for the localization and tandem affinity 
            purification of protein complexes from metazoans.
  JOURNAL   Sci STKE. 2005 Jan 11;2005(266):pl1.
  PUBMED    15644491
REFERENCE   2  (bases 1 to 16397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16397
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     668..684
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(9952..9968)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10022..10050)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10798..10818
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             10904..10924
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10937..10981
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        complement(14206..14224)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14245..14261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14245..14261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14258..14280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14269..14285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(14293..14323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(14476..14493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14647..15235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(15406..16266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16029..16048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16267..16371)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      661 cccccctcga ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat tttatagttt
      721 tttttctgtt tgaaaaattc tccccatcaa tgttctttca aataaataca tcactaatgc
      781 aaagtattct ataacctcat atctaaattc ttcaaaatct taacatatct tatcattgct
      841 ttaagtcaaa cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc tctcaaaatt
      901 cagtttttta aaccactcct atagtcctat agtcctatag ttacccatga aatccttata
      961 tattactgta aaatgtttca aaaaccattg gcaaattgcc agaactgaaa atttccggca
     1021 aattggggaa ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg taattgccga
     1081 aagtttttga cacgaaaatg gcaaattgtg gttttaaaat tttttttttt ggaaatttca
     1141 gaatttcaat tttaatcggc aaaactgtag gcatcctaag aatgttccta catctatttt
     1201 gaaaagtaag cgaattctat gaaaatgtct aaagaaaatg gggaaacaat ttcaaaaagg
     1261 cacagtttca agtgtttccg aattatacta aatccctcta aaaacttccg gcaaattgat
     1321 atccgtaaaa gagcaaatcc gcatttttgc cgaaaattaa aatttccgac aaatcggcaa
     1381 accggcaatt tggcgaaatt tgccggaagg caattgccgc ccacccctgt tccagaggtt
     1441 caaactggta gcaaagctca aaatttctca aattctccaa tttttttttg aattttggca
     1501 gtgtaccaaa atgacattca gtcatattgg tttattatag atttatttag ataaaatcct
     1561 aaatgattct acctttaaag atgcccactt taaaagtaat gactcaaact tcaaattgct
     1621 ctaagattct attgaattac catcttttcc tctcattttc tctcactgtc tatttcatca
     1681 caaattcatc cctctctccc tctctttctc tctccctctc tctctttctc tttgctcatc
     1741 atctgtcatt ttgtccgttc ctctctctgc gccctcagcg ttccccacac tctctcgctt
     1801 ctcttttcct agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc attttccatc
     1861 tctgtcaatc attacggacg acccccatta atttttggat gtgctctgcg agcaagagca
     1921 cacggaactc atttattttt tgttggaatt tctttttcat tttagttagt taaaaggtta
     1981 ttcagtgcaa aaattgattt tttgctcttt tggtacatta gaggggtttg aaagttttcc
     2041 ataaaaaggg tttccggagt aaaaaatcta aaaattttta caaacatttc caaaaatttt
     2101 gggaacatat cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt taatcaatta
     2161 attggatgcg ttcaaatatt ttaaaaatat ttttgaattg catatttctt tactcataaa
     2221 aaaattatta atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa actgtaaatc
     2281 ccataaaaca gtgttatttt ttagttattt ttagtaatta tttgtgaatt aaaaacacat
     2341 tttttttcaa aatattcctt gaataaataa aattgaaagt gaattcaaaa tatttcacca
     2401 attttacaaa atttgaaaat ttgaaaaaat attttctacc aaattgtgct caaaaaatat
     2461 tattaagttc ccaggaaatt tatgatgaaa acttggaata tttttggtaa aattttctca
     2521 aaaaattgaa agttgtcaaa aacacactaa cactatacct cgcggcatag aaaaaactgg
     2581 tggccgaatt ttttaaacca atttaaatta aatatatttt tgcactttgt acaaaaaaat
     2641 ttgaagttcc caggaaattc atacggaaat attttccaga acttttataa tttttatttt
     2701 aattctcaaa atttacaata aaaacttgga aacctgaaat ttgcttaaag aataacccta
     2761 tcccccacgg cctagaaaat actggtggcc gaattttttc cgcagccaca ccacctctaa
     2821 tctccagagc actctccaaa attcccataa tccccaaaat tttcagctac aacagcatat
     2881 atgaaggcag agcaacaaca acaatcgatc gcacccggct cggcaacctt cccgtctcag
     2941 gtgaggaaga cgcagagaaa tgagagagag agcgtgttta tgcacgcagt atactaactc
     3001 ctgatatcca atttcatata tcgatatgct tgctttcttt tcttctacgt tgaaattgtt
     3061 tgaacttttc tcaaaaaacg cggattttag agagacttag agttgctcca aataaattta
     3121 atttgcaatt ttgcacttac tattagaatc tacttttata gcgctaaaat gaccgaataa
     3181 cacagtagat tttaaaagaa aattttaaaa attatttttt tgtgcagtca aatttcgcta
     3241 gtttttgcca ctcatttttg aacatttgta tatgtgcctt tcggtggttc aatcaggggt
     3301 ggcacgtggt gtcagagtgt tccatttcgg tttgatctac gttgatctac aaaaaatgcg
     3361 ggagaagaga cgcagagttc tcaactgatt tcacatggtt atgaaccagg ggtgttcggc
     3421 aaatttgccg tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc cttttatgaa
     3481 cgtgctgacg tcacatcttt ttggacaaag aattcccgca ttttttgtat atcaaaccgt
     3541 gatgggacag cctggcacca cgtggggttg gcggcaaatg attttttccg acaaatcggc
     3601 aaattggcaa attgccggaa tttaaagttt ctggcaaatt gttgatttgc cgaaaaaaaa
     3661 tttcaaaaag gcacagtttt aagtgtttcc gtcttataaa atatttctct aaaaatttcc
     3721 ggaaaacgga aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc tgcaatttgc
     3781 cgaacggcaa ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt tgaatattaa
     3841 aaaaaaaatt cacacaaaaa taataaattg aaataggcta aattcacatc tatgtacaaa
     3901 aattgcatgc cagcaccggt caattttctc aattactttt tgtctatttt tgccgaacat
     3961 aatttgctgt ttccttggca gaatacagaa aactcttttt tttttcagat gccccgaccc
     4021 ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt
     4081 acaccaaatg atgtgctagc tcttccagga atcactcaag gtaagtgaat atttgctcta
     4141 ttgataaacg cgccgtgtac tccacgtgga caaacatatc aaattttcag gattcctatg
     4201 ctccccatcg gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga
     4261 cacggaacac gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca
     4321 ggcgcaagcc gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt
     4381 aaagacggtc ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg
     4441 aatgattgaa cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg
     4501 attctgtatg ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc
     4561 acaacaactc agtgagtttg aagcgggaaa aattatttga gctcattttg tcaatagagc
     4621 gaattttcta ggtttttaaa aataaaataa atattgacaa aaagctgccc tacagtagtt
     4681 caaaatgtga aaacttggag gaaattcgag ttttctctga gaagtttttg gcgggtaatt
     4741 caaattttct gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa aattatttga
     4801 cgggaaattc aaaatttctg gaaatgtatt gaacatttga gaaattttgt taaaaactac
     4861 attaaaccga taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca tcgaagagaa
     4921 gaattcttgc caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa actgactaat
     4981 tggaaagtaa aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa aattaaaaaa
     5041 aaaactgtat attttcagtg gacgacatga tcaataatcc aaacgagacc cgctccgata
     5101 gcttctattt tgtggagaat aagctcgtaa tgcacaataa ggccgactac tcgtatgatg
     5161 cataatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg
     5221 tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg
     5281 cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa
     5341 atataatgtt ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa
     5401 aaaacttgaa ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa
     5461 atttggaaaa tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga
     5521 tttgccgatt agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa
     5581 atttccaatt ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa
     5641 acttctcaac aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag
     5701 ttccggcatc taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat
     5761 ttgccgcttt gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca
     5821 ttccaattcc gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt
     5881 tgccgatttg ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt
     5941 tgcaaaagat aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca
     6001 tccaaaacac cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag
     6061 gagaagcctc cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac
     6121 catttgaatg gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa
     6181 agctgaagat actgatatta tatgtatatt tcattattct ttttccacaa aattttccga
     6241 catttaattc cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt
     6301 gtcttcttct tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt
     6361 cggcttaaca aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggcg
     6421 gccgcgcgga agatctggaa gtaaaatatt gtaatatttt ccctaaattt cacttaaaaa
     6481 taaggatttt tgcaacgtaa aacctcaaaa attcgctgaa attgacgatt tttgttcaaa
     6541 attttgaaaa acagggttaa ttttagctga aaatcttcaa ttttgccaga aattggcttt
     6601 ttttccgtcg ccacacggag aacttgccaa aaatgaagac ttttagccaa tttagccctg
     6661 aaaattcgag ttttgggcga tttttggtct gagaagccgt tttcgcagta tattatattt
     6721 tctcgttaaa tttcatttta aaataaacat tttgaacgta taaccccaaa aattcgctgc
     6781 gcaatggatg atttttgtcc aaagttttga taattttagc taaaaacctc gaattttggc
     6841 aatttttcgt gccgcatcgg ccgaaagttg actttttttg gaattatcat aatttaatgc
     6901 acattttggt agtttatctc acataatctg tctgcaggcg ggaaaaattt gagataaact
     6961 actaaaagat gcaaaacaag gatgataatt ccagaaaatc aacttcggac cgtctcgaca
     7021 cggaaaattg agcgaaattg gagttttaaa atgatttagc cctgaaaatt cgagaaattt
     7081 aggcggtttt gggtctgaaa aggcgttttc gcagtatttt ctcgctaaat ttcatttatt
     7141 atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt gttcgataat
     7201 tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt tttcccttgt
     7261 aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa gcaactcaaa
     7321 aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct aaatagtgat
     7381 ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg aaaatttgtc
     7441 aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa ttgaaagttt
     7501 tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa ttctgagaat
     7561 gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc gaaaactaca
     7621 gtaatcctta aatgactact gtagcgattg tgtcgattta cgggctcgat tctcggaaaa
     7681 aaaaatcatt tttcaaattt tgacagcgat attcaatttt tcttcgtttt ttcgtatttc
     7741 tcagtcattt ttgtgcttat ttttaatatt ttattcatta ataaattaat ttaattgaaa
     7801 aacgtccaga cgtattttta catttcccgt ctatttccgt aataggcggt taataacgta
     7861 gaaatacgtc tgtatgagtt tactcatcaa aatgacacga atattggacg tttttaaatc
     7921 aaattaattt attaataggt aaaatattaa aaaataaaca caaaaatgac aaaggaatag
     7981 gaaaacacga agagaaattg agtatcgctg tcaaaattcg aaaaacgtgg atttttccaa
     8041 gaatcgagcc cgtaaatcga cacattcgct acagtagtca tttaaggatt attgtagttt
     8101 tcgctacgag atactttgcg cgccaaaaac gtggtgaaat acgcattctc agaattttat
     8161 gttcccgtaa taccacaatg accgaatatc ataataaaaa aattcaaaaa caatttctag
     8221 attttatatg attttctgaa aattgaaaaa atctcagttt tcacctaatt atatttgaac
     8281 tacagccaat tgaactcgtt cgttggagcg cgcttgcatt attttcatta attaatttta
     8341 ttaatcctca ttatttcact gattttcttc atttttgagg ttttttttat cgggaaatga
     8401 aagaaatata caagaaaaat gcaaaatgtt tattaaaaac tgaaattagt atttctcgga
     8461 gttttaggca ttttcagtta ctttttaata aagattttgc atttttcttg tatatttctt
     8521 tcattttccg attaaaaaac cccaaaaatg aagaaaatca gtgaaataat gaaaattaat
     8581 tcaaataact aatgaaaata atgcaagcgc gctccaacca acgagttcaa ttggcggtaa
     8641 ttcaaatata attaggtgaa aactgagatt ttttcaattt tcaaaaaatc atataacatc
     8701 tagacaattt ttttgaattt ttttatcatg atattcggtc attgtgtccc cataggcatg
     8761 ttttaaagca atttccacct ttaaataaaa tcgagaaaaa atggcgtcaa aagacatatg
     8821 taaaagctgt ttctctttgt atattcacac agaatatagc ccgattttgg aggtgaaaaa
     8881 ccggtttttt tgtattcttt acgccacata aagtgatatg gaagagaaaa tcgacatatt
     8941 tatgtacttg gagtacgagt acattggaga aaaagggtga actactggaa aatctgggaa
     9001 atttcagaaa aaaattccga aaaatctttt ttgctgaaaa aaaaatttgt tcgaaatttg
     9061 ggaaatactg gaaaattttc atcaaaatta aagcgatttt taacgttttt gcttgggaga
     9121 tactatttgt aattttaatg tttggtgagg acaacagaaa tgaccttcag cgtacggaaa
     9181 acgattaaaa gacacatttg aatcgaaaat agcgtgaaaa acatgaaaat atcggaaaaa
     9241 tagctttaaa attggatttg aaagcaaaaa atgtatcaaa aatcgagaaa aatgcttgaa
     9301 aaagttgaaa aactcaaaca aaaaatcaaa aaaggttttt ttaaaaatct gtgagtatcg
     9361 caacgaaagc acaacttgag gcatgcgcct ttaaaccaac cgtaaccaaa ttcggaggca
     9421 aaaaaagatt tctcgctgtt ttttcagttt taaaatcact tttcacgttt cttttcgcag
     9481 taattcgttt gcaaaacatc tgaaaaaatt ttattttata agaaattata taaaaatcgc
     9541 tataaattga gtttttgccc ccaaattcag cacggagcac ttctcaatta aatataattt
     9601 tattttttaa cattgaaagc tttcataaaa ttgagctttg tttgtaaata atcagtgaaa
     9661 aaacacagaa atcttcaaaa aataaagaaa aagtttttaa aaatctgtga gtcccgcaac
     9721 gaaaataaaa ctttaggcat gcgcctttaa accaaccgta accaaattcg gcggcaaaaa
     9781 tggatttctc gccgtttttt cagttttaca atcacttttt aggtttcttt ttgcagtatt
     9841 tcgtttccca aacaattaaa aatcaaattt tcttttccag atggctcaaa caaagccgat
     9901 tgccgagcaa atgggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt
     9961 cgacctcgag ggggcggtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg
    10021 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
    10081 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
    10141 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
    10201 tttaaatttt cagccaacac ttgtcactac tttctgttat ggtgttcaat gcttctcgag
    10261 atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
    10321 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca
    10381 gttcggtact aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg
    10441 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
    10501 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
    10561 acaaaagaat ggaatcaaag ttgtaagttt aaacatgatt ttactaacta actaatctaa
    10621 tttaaatttt cagaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
    10681 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
    10741 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
    10801 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaatg
    10861 cccgggggat cggtggagct ccaccggtgg cggccgctct agagagaatc tttattttca
    10921 gggcgccgcc aaattcaaag aaaccgctgc tgctaaattc gaacgccagc acatggacag
    10981 cggaggtgga ggtactagtg gatcctaatt ttgccgtatt ttccatattt tgttttgtat
    11041 atttatccac tcaccccctc tctttgtcct gtgaatgaac ttgtgccaaa aaagccaaaa
    11101 ataatttact ttttttaaag acatattaaa acttgaaaac ttggaacaat acggtgcgaa
    11161 agcgaaacag agacagaaaa atgaacaaag agatccgtga aaagtgaacg atgatttagg
    11221 cttcttgttg ggcggcgtgg atgagagtga tgttgtctcc tttgagaaga attctgccga
    11281 tcttgttgcg tcccttcgtc ttcatgttca cctcctcggc ttcgtcgaac acgacattca
    11341 tgaactcgtc gaatccgatg atgtagccct cgagacggtg ggtgacatcc tcgtacaacc
    11401 agatttggac ccgagtgcgg ttttggagat atctgaaatt tgggaaattg ttttctactg
    11461 attagtgaaa aaatattacc tgaagatgag gttcacaggc tgaaccatca ctttgttgag
    11521 ctttctcgtc gacatttttg cgggatgatc tgtaaaaatc aatatatttt ttgaaagaca
    11581 caaattttta agataaatat caagaaaact cgattttagc agctatttaa gctcaaaaac
    11641 tacaatttat ggctttaaaa cataattttt cacattttac ataaatttca acgaaaaaaa
    11701 atgccaaaaa acttcaaatt tgagcagaaa tcggctattt tctcgacaaa aataatttcg
    11761 aaaattcgtt caaaaaagat gagtacgcaa gcaccgtgac gcacagttgc ggactttgta
    11821 aaccgtaccc ctgcataaaa aggcggaatt ttgaatttaa taaaatgcag tttagaacaa
    11881 agaaaagcac ggagagaatt gtgataacta attatttgta tttaatttta aatactacat
    11941 aaaactgaag attggaacga gttaaagctg gtatttgcgg tttatagccc caaaagttaa
    12001 ggcttgttag gcaattatcg ataaaaacaa cgagaaattc gtgaaatcgg caaaaattta
    12061 tagaaaacgt ttgaattaaa ttaaatttac gcttttgccg ccaacaccta tcgagaccct
    12121 agttataggg gcggagcgtc atgggagcgc gcttgcaact cgatttcaaa aagtttcgct
    12181 gtttgtacaa cattctcagt tgttcttaat cagttaaata agtttcaaaa ttttcaaaat
    12241 cataaaacac agttaatgct aaattaaagt ctaaaaaatc aggtgataag gggtttggac
    12301 gaccaaaaag cgaccttcgc tcgaaaattg ataaactaac tacacttttt ccggaaaaat
    12361 gatggaaaaa tcctgatttt cttcgttgaa tcaatttttg tttcattttt aacactttca
    12421 catgaaatat ttcagttaaa atgctcaagc taattgggtt tcttataatt ttgggtctcg
    12481 caacggccga caagtgctca gattgtttgg ctcaggggaa acagtgagtt ggaagcttac
    12541 aattctgaga attcgtattg cgcagcttat ttgacgcgca aaatatgaaa tatcacgtag
    12601 cgaaaactac agtaattctt taaataagac tactgtagtg cctatgtcga tttactgcct
    12661 caactttctt aatcaagctc gtaaatcgac accagctcta cagtagtcat agttttcgct
    12721 acgagatatt ttgcgcgtca aatatggtgt gcaatacgca ttttcagaat tttatgttct
    12781 cgtaatatac cttttttcag ctggtgtgtt actgtaaatg catgtggatt tacgccatgt
    12841 gtcactacaa ttacagtgag tttttttgaa gaaaaaaaaa ttacccattt gatcgccaat
    12901 tccatttttc aaaaatcaat aaaaaaaaaa aatttcggcg atcagaaacg ttacagaaag
    12961 ccctaaactg cccggaacta ccagataaag cccacgctta cgatgattca tttatgagga
    13021 ataaggtatt gatcacacat tctgctgcct acaatatgga cccacaaaag tgttttacgt
    13081 aggttcacct tctagataaa ccttaaaaat cgatactttc agtagtaaaa tgccttcgat
    13141 gaaagtttcc aaaacagtta atgtcaattg tgatagatat gggccaagtg agttgtttta
    13201 ttagcccacg gatttctggc ttccctcata aattgaaaag gaagagattg ccgaactaga
    13261 ccattttggc tcggcgcgtt gcgtgtcacg tcgcggctcg attgtaaaac taaatgtatt
    13321 ggtccgtgtg gagtacacga ctttcccttt ccggcaggcg attgtcaatg gagcgcgaaa
    13381 aattcaatga ggaaggccag aaccccgtgt ctgtagtttt ctcaaaaagt ggcaaaaact
    13441 caataattgc caatttttga ctgccaataa aaatttcact aaatttatta aatttatttt
    13501 aagttttatt gaaatttttt taaaatttcc ccactggcgc taaaaaatcc aattttcaga
    13561 agcaatgtgc ttcagcttca cggccataga tactgtccaa aaagtgattg caatgacgtt
    13621 tagagggaca gaaggagcag ctcaattgtc tgaagaaatt ttggatttct tccagtaagt
    13681 tcgggaaatg gcaatttaaa gtttaattgg atttttgtat tttttcttct cacgactaca
    13741 gttttgcaca tttttatgtt ataaaaaacc aaaaaaaaat ttttttgcct tttttctcag
    13801 cttaccacaa aacaatttcg accaaaaata aattgaaaaa attaaaaaat tttttgcacg
    13861 gttttcggac tcaaaaaaag ctgaatgttc ctggaactat ttgtaaaatt caaaattcaa
    13921 aattttcacg aattctctaa aaggttccag caaattccac aactttcttg aatttttcaa
    13981 aaaagttctt gagcattcaa caattatttt tggaattttc caaaggttac aggacgttcc
    14041 aatttttcaa aaaaatttct cgcaaaaaat gttttctcag aaaatttgaa tttccctcca
    14101 cataaaagat tgttctcaaa aaatttgcaa acacacattt tcagcggaaa aaaggcgttc
    14161 ttcgactctg gaagtgtatt cgaatatggt acccagcttt tgttcccttt agtgagggtt
    14221 aattccgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    14281 cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    14341 agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    14401 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    14461 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    14521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    14581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    14641 ggcgtttttc cataggctcg gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    14701 gaggtggcga aacccgacag gactataaag ataccaggcg ttcccccctg gaagctccct
    14761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    14821 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    14881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    14941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    15001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    15061 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    15121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    15181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    15241 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    15301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    15361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    15421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactgcc
    15481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    15541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    15601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    15661 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    15721 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    15781 acgatcaagg cgagttacat gatcccccat gttgtgaaaa aaagcggtta gctccttcgg
    15841 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgc ttatggcagc
    15901 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    15961 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    16021 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    16081 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    16141 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    16201 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    16261 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    16321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    16381 ccgaaaagtg ccacctg
//
