LOCUS Exported 2406 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pFSBC18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2406) AUTHORS Chubiz LM, Lee MC, Delaney NF, Marx CJ TITLE FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. JOURNAL PLoS One. 2012;7(10):e47959. doi: 10.1371/journal.pone.0047959. Epub 2012 Oct 31. PUBMED 23118913 REFERENCE 2 (bases 1 to 2406) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2406 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 173..189 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(235..254) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 354..376 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(414..432) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 500..604 /gene="bla" /label=AmpR promoter CDS 605..1465 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(823..842) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1636..2224 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2125..2144 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2378..2395 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaaaa tgatacggcg 121 accaccgaga tctacactct ttccctacac gacgctcttc cgatctgata gcgtaaaacg 181 acggccagtt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 241 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 301 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 361 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 421 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 481 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 541 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 601 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 661 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 721 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 781 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 841 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 901 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 961 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 1021 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 1081 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 1141 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 1201 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 1261 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 1321 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 1381 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 1441 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 1501 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 1561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 2221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 2341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 2401 ggaaga //