LOCUS Exported 4732 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection. ACCESSION . VERSION . KEYWORDS pFA6a-link-yoPSmOrange-SpHis5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4732) AUTHORS Lee S, Lim WA, Thorn KS TITLE Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. JOURNAL PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PUBMED 23844123 REFERENCE 2 (bases 1 to 4732) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4732 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 18..205 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(73..97) /label=pHybLex-R /note="ADH terminator, reverse primer" gene 280..1480 /label=HIS3MX6 /note="yeast selectable marker encoding the S. pombe his5 gene, which corresponds to S. cerevisiae HIS3" promoter 280..623 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 624..1277 /codon_start=1 /gene="Schizosaccharomyces pombe his5" /product="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /label=S. pombe his5 /note="yeast auxotrophic marker; corresponds to S. cerevisiae HIS3" /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL" terminator 1283..1480 /label=TEF terminator /note="Ashbya gossypii TEF terminator" primer_bind complement(1584..1603) /label=T7 /note="T7 promoter, forward primer" promoter complement(1585..1603) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1690..1707) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1861..2449) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1941..1960) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2620..3480) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3243..3262 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3481..3585) /gene="bla" /label=AmpR promoter primer_bind 3653..3671 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(3709..3731) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 3831..3850 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 3931..3949 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 3931..3948 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta 61 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct 121 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg 181 ctcttattga ccacacctct accggcagat ccgctaggga taacagggta atatagatct 241 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc 301 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt 361 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg 421 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca 481 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg 541 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac 601 atcacatccg aacataaaca accatgggta ggagggcttt tgtagaaaga aatacgaacg 661 aaacgaaaat cagcgttgcc atcgctttgg acaaagctcc cttacctgaa gagtcgaatt 721 ttattgatga acttataact tccaagcatg caaaccaaaa gggagaacaa gtaatccaag 781 tagacacggg aattggattc ttggatcaca tgtatcatgc actggctaaa catgcaggct 841 ggagcttacg actttactca agaggtgatt taatcatcga tgatcatcac actgcagaag 901 atactgctat tgcacttggt attgcattca agcaggctat gggtaacttt gccggcgtta 961 aaagatttgg acatgcttat tgtccacttg acgaagctct ttctagaagc gtagttgact 1021 tgtcgggacg gccctatgct gttatcgatt tgggattaaa gcgtgaaaag gttggggaat 1081 tgtcctgtga aatgatccct cacttactat attccttttc ggtagcagct ggaattactt 1141 tgcatgttac ctgcttatat ggtagtaatg accatcatcg tgctgaaagc gcttttaaat 1201 ctctggctgt tgccatgcgc gcggctacta gtcttactgg aagttctgaa gtcccaagca 1261 cgaagggagt gttgtaaaga gtactgacaa taaaaagatt cttgttttca agaacttgtc 1321 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta 1381 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat 1441 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg 1501 ccgccatcca gtttaaacga gctcgaattc atcgatgata tcagatccac tagtggccta 1561 tgcggccgcg gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt 1621 taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 1681 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 1741 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 1801 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 1861 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 1921 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 1981 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 2041 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 2101 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 2161 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 2221 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 2281 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 2341 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 2401 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 2461 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 2521 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 2581 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 2641 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 2701 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 2761 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 2821 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 2881 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 2941 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 3001 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 3061 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 3121 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 3181 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 3241 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 3301 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 3361 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 3421 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 3481 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 3541 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 3601 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 3661 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 3721 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 3781 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 3841 gcagattgta ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta 3901 atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa cgcggccgcc 3961 agctgaagct tcgtacgctg caggtcgacg gatcggtgac ggtgctggtt taattaacat 4021 ggtgtctaaa ggtgaggaaa acaacatggc aatcattaag gaattcatga gattcaaagt 4081 cagaatggaa gggacagtta atggccacga gtttgaaatc gaaggcgaag gtgaaggtcg 4141 tccatacgag ggttttcaaa cagctaagtt aaaagtgacg aaaggaggac cactaccatt 4201 cgcttgggat attctcagtc cattgtttac ttatgggtca aaagcttacg ttaaacatcc 4261 tgccgacatc cctgactact ttaagttgtc cttcccagaa gggtttaagt gggaaagagt 4321 tatgaattat gaggacggag gtgttgtaac tgtcacccaa gactcttctc ttcaagatgg 4381 tgaattcatc tacaaagtta agatgagagg cacaaatttt ccaagcgatg gcccagttat 4441 gcagaaaaag acaatgggct gggaggcatc ctcagagagg atgtaccctg aagatggagc 4501 gttaaaggga gagatacgaa tgagactgaa gttaaaggat ggaggtcatt acactagtga 4561 agtaaaaact acttataaag ctaaaaagtc tgtacaactt cctggcgcat atattgtggg 4621 tattaaacta gatataacat cacacaatga agattacacc atcgtcgaac aatacgaaag 4681 agccgaaggt agacattcta caggtggtat ggatgaattg tacaaataag gc //