LOCUS Exported 978 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown. ACCESSION . VERSION . KEYWORDS pdCas9-humanized SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 978) AUTHORS Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA TITLE Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. JOURNAL Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. PUBMED 23452860 REFERENCE 2 (bases 1 to 978) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..978 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 40..49 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 cagccctcac tccttctcta ggcgccggaa ttagatctcg ccaccatgga caagaagtat 61 tctatcggac tggccatcgg gactaatagc gtcgggtggg ccgtgatcac tgacgagtac 121 aaggtgccct ctaagaagtt caaggtgctc gggaacaccg accggcattc catcaagaaa 181 aatctgatcg gagctctcct ctttgattca ggggagaccg ctgaagcaac ccgcctcaag 241 cggactgcta gacggcggta caccaggagg aagaaccgga tttgttacct tcaagagata 301 ttctccaacg aaatggcaaa ggtcgacgac agcttcttcc ataggctgga agaatcattc 361 ctcgtggaag aggataagaa gcatgaacgg catcccatct tcggtaatat cgtcgacgag 421 gtggcctatc acgagaaata cccaaccatc taccatcttc gcaaaaagct ggtggactca 481 accgacaagg cagacctccg gcttatctac ctggccctgg cccacatgat caagttcaga 541 ggccacttcc tgatcgaggg cgacctcaat cctgacaata gcgatgtgga taaactgttc 601 atccagctgg tgcagactta caaccagctc tttgaagaga accccatcaa tgcaagcgga 661 gtcgatgcca aggccattct gtcagcccgg ctgtcaaaga gccgcagact tgagaatctt 721 atcgctcagc tgccgggtga aaagaaaaat ggactgttcg ggaacctgat tgctctttca 781 cttgggctga ctcccaattt caagtctaat ttcgacctgg cagaggatgc caagctgcaa 841 ctgtccaagg acacctatga tgacgatctc gacaacctcc tggcccagat cggtgaccaa 901 tacgccgacc ttttccttgc tgctaagaat ctttctgacg ccatcctgct gtctgacatt 961 ctccgcgtga acactgaa //