LOCUS Exported 514 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Bacterial expression of mKalama1 blue fluorescent protein. ACCESSION . VERSION . KEYWORDS pBad-mKalama1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 514) AUTHORS Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE TITLE Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. JOURNAL Biochemistry. 2007 Apr 20. ():. PUBMED 17444659 REFERENCE 2 (bases 1 to 514) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..514 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 66..88 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 108..125 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 129..161 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 174..194 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" ORIGIN 1 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagaa 61 ataattttgt ttaactttaa gaaggagata tacatatgcg gggttctcat catcatcatc 121 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgagaacc 181 tgtacttcca gggctcgagc atggtgagca agggcgagga gctgttcacc ggggtggtgc 241 ccatcctggt cgagatggac ggcgacgtaa acggccgcaa gttcagcgtg cgcggcgtgg 301 gcgagggtga tgccacccac ggcaagctga ccctgaagtt catctgcacc tccggcaagc 361 tgcccgtgcc ctggcctacc ctcgtgacca ccctgtctta cggcgtgcag tgcttctccc 421 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 481 tccaggagcg caccatcttc ttcaaggacg acgg //