LOCUS Exported 922 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Lentiviral shRNA vector (pGIPz based) for inducible knockdown. ACCESSION . VERSION . KEYWORDS pInducer10-mir-RUP-PheS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 922) AUTHORS Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, Elledge SJ TITLE The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. JOURNAL Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. PUBMED 21307310 REFERENCE 2 (bases 1 to 922) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..922 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 57..752 /codon_start=1 /product="red fluorescent protein from Entacmaea quadricolor" /label=TurboRFP /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC DLPSKLGHR" ncRNA 803..896 /label=5' miR-30a /note="sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)" /ncRNA_class="miRNA" primer_bind 896..912 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 897..913 /label=KS primer /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gctgttttga cctccataga agacaccggg accgatccag cctaccggtc gccaccatga 61 gcgagctgat caaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc 121 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga 181 agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct 241 tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc 301 agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc 361 tgaccgctac ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca 421 acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg 481 ccaacaccga gatgctgtac cccgctgacg gcggcctgag aggccacagc cagatggccc 541 tgaagctcgt gggcgggggc tacctgcact gctccttcaa gaccacatac agatccaaga 601 aacccgctaa gaacctcaag atgcccggct tccacttcgt ggaccacaga ctggaaagaa 661 tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaagtact 721 gcgacctccc tagcaaactg gggcacagat gatgcggccg caagccttgt taagtgctcg 781 cttcggcagc acatatacta tgttgaatga ggcttcagta ctttacagaa tcgttgcctg 841 cacatcttgg aaacacttgc tgggattact tcttcaggtt aacccaacag aaggctcgag 901 gtcgacggta tcgataagct tg //