LOCUS       Exported                 733 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pID3.01B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 733)
  AUTHORS   D'Agostino I, Merritt C, Chen PL, Seydoux G, Subramaniam K
  TITLE     Translational repression restricts expression of the C. elegans 
            Nanos homolog NOS-2 to the embryonic germline.
  JOURNAL   Dev Biol. 2006 Apr 1. 292(1):244-52.
  PUBMED    16499902
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    74..198
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        223..253
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(373..392)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 catggatgaa ctatacaaat gcccggggga tcggtggagc tccaccggtg gcggccgctc
       61 tagaactaga tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
      121 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
      181 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
      241 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
      301 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      361 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      421 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
      481 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
      541 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      601 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
      661 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
      721 attgagaata tgt
//
