LOCUS Exported 903 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pDN-D2irTNG4kwh SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 903) AUTHORS Nevozhay D, Zal T, Balazsi G TITLE Transferring a synthetic gene circuit from yeast to mammalian cells. JOURNAL Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471. PUBMED 23385595 REFERENCE 2 (bases 1 to 903) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..903 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 215..233 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 241..259 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind complement(284..304) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 459..838 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 aagaaagaac aatcaagggt ccccaaactc accctgaagt tctcaggatc gatccggagc 61 tttttgcaaa agcctaggcc tccaaaaaag cctcttcact acttctggaa tagctcagag 121 gccctagagg atccccgggt accaagctta agtttaaacg ctagagtccg gaggctggat 181 cggtcccggt gtcttctatg gaggtcaaaa cagctctcta tcactgatag ggagatctga 241 tctctatcac tgatagggat gcttatatag acctcccacc gtacacgcct accgcccatt 301 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa 361 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc 421 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat 481 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag 541 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga 601 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc 661 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg 721 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg 781 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa 841 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc 901 ttc //