LOCUS Exported 806 bp ds-DNA linear SYN 03-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pFSBC2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 806) AUTHORS Chubiz LM, Lee MC, Delaney NF, Marx CJ TITLE FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. JOURNAL PLoS One. 2012;7(10):e47959. doi: 10.1371/journal.pone.0047959. Epub 2012 Oct 31. PUBMED 23118913 REFERENCE 2 (bases 1 to 806) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..806 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 194..211 /label=L4440 /note="L4440 vector, forward primer" primer_bind 394..411 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 395..411 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(457..476) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 576..598 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(636..654) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 61 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 241 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 301 gaaagcgggc agtgagcgca acgcaattaa aatgatacgg cgaccaccga gatctacact 361 ctttccctac acgacgctct tccgatctcc tgttgtaaaa cgacggccag ttacgcatct 421 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata 481 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 541 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 601 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 661 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 721 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 781 acaataaccc tgataaatgc ttcaat //