LOCUS       Exported                 911 bp ds-DNA     linear   SYN 10-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pIC113
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Cheeseman IM, Desai A
  TITLE     A combined approach for the localization and tandem affinity 
            purification of protein complexes from metazoans.
  JOURNAL   Sci STKE. 2005 Jan 11;2005(266):pl1.
  PUBMED    15644491
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             98..142
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     misc_feature    161..213
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    337..458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(374..393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     428..447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(602..623)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     814..833
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catggacgag ctgtacaagt ccggagggga tcggtggagc tccaccggtg gcggccgctc
       61 tagagagaat ctttattttc agggcgccgc caaattcaaa gaaaccgctg ctgctaaatt
      121 cgaacgccag cacatggaca gcggaggtgg aggtactagt ctcgagctca agcttcgaat
      181 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
      241 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
      301 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
      361 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      421 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
      481 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
      541 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
      601 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
      661 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
      721 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
      781 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
      841 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
      901 tgcgccgcta c
//
