LOCUS Exported 940 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION 3rd generation lentiviral backbone. ACCESSION . VERSION . KEYWORDS pRRLSIN.cPPT.PGK-GFP.WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 940) TITLE Trono Lab Misc Plasmids REFERENCE 2 (bases 1 to 940) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..940 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 26..35 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(51..561) /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind complement(114..132) /label=hPGK-F /note="Human PGK promoter, forward primer" misc_feature 607..724 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcccc ctggggagag 61 aggtcggtga ttcggtcaac gagggagccg actgccgacg tgcgctccgg aggcttgcag 121 aatgcggaac accgcgcggg caggaacagg gcccacacta ccgccccaca ccccgcctcc 181 cgcaccgccc cttcccggcc gctgctctcg gcgcgccctg ctgagcagcc gctattggcc 241 acagcccatc gcggtcggcg cgctgccatt gctccctggc gctgtccgtc tgcgagggta 301 ctagtgagac gtgcggcttc cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct 361 tcccgactta ggggcggagc aggaagcgtc gccggggggc ccacaagggt agcggcgaag 421 atccgggtga cgctgcgaac ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg 481 cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg gctgccttgg 541 aaaaggcgca accccaaccc cgtggaattc gatatcaagc ttctcgaggc tagtctcgtg 601 atcgataaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt 661 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt 721 aaaagttaac cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct 781 ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg 841 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc 901 aaaaactatt cttaaaccta ccaagcctcc tactatcatt //